Please use this identifier to cite or link to this item:
Title: Autophagy and oxidative stress associated with gold nanoparticles
Authors: Li, J.J.
Bay, B.-H. 
Hartono, D. 
Yung, L.Y.L. 
Ong, C.-N. 
Keywords: Autophagosomes
Gold nanoparticles
Lung fibroblasts
Oxidative stress
Issue Date: 2010
Citation: Li, J.J., Bay, B.-H., Hartono, D., Yung, L.Y.L., Ong, C.-N. (2010). Autophagy and oxidative stress associated with gold nanoparticles. Biomaterials 31 (23) : 5996-6003. ScholarBank@NUS Repository.
Abstract: Elemental metal nanoparticles like cadmium and silver are known to cause oxidative stress and are also highly toxic. Yet for gold nanoparticles (AuNPs), it is not well established whether these particles are biologically toxic. Here we show that AuNPs, which were taken up by MRC-5 human lung fibroblasts in vitro, induce autophagy concomitant with oxidative stress. We also observed formation of autophagosomes together with the uptake of AuNPs in the lung fibroblasts as well as upregulation of autophagy proteins, microtubule-associated protein 1 light chain 3 (MAP-LC3) and autophagy gene 7 (ATG 7) in treated samples. AuNP treated cells also generated significantly more lipid hydroperoxides (p-value < 0.05), a positive indication of lipid peroxidation. Verification with western blot analysis for malondialdehyde (MDA) protein adducts confirmed the presence of oxidative damage. In addition, AuNP treatment also induced upregulation of antioxidants, stress response genes and protein expression. Exposure to AuNPs is a potential source of oxidative stress in human lung fibroblasts and autophagy may be a cellular defence mechanism against oxidative stress toxicity. © 2010 Elsevier Ltd.
Source Title: Biomaterials
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2010.04.014
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.