Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2010.04.014
DC FieldValue
dc.titleAutophagy and oxidative stress associated with gold nanoparticles
dc.contributor.authorLi, J.J.
dc.contributor.authorBay, B.-H.
dc.contributor.authorHartono, D.
dc.contributor.authorYung, L.Y.L.
dc.contributor.authorOng, C.-N.
dc.date.accessioned2011-07-25T02:43:35Z
dc.date.available2011-07-25T02:43:35Z
dc.date.issued2010
dc.identifier.citationLi, J.J., Bay, B.-H., Hartono, D., Yung, L.Y.L., Ong, C.-N. (2010). Autophagy and oxidative stress associated with gold nanoparticles. Biomaterials 31 (23) : 5996-6003. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2010.04.014
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24365
dc.description.abstractElemental metal nanoparticles like cadmium and silver are known to cause oxidative stress and are also highly toxic. Yet for gold nanoparticles (AuNPs), it is not well established whether these particles are biologically toxic. Here we show that AuNPs, which were taken up by MRC-5 human lung fibroblasts in vitro, induce autophagy concomitant with oxidative stress. We also observed formation of autophagosomes together with the uptake of AuNPs in the lung fibroblasts as well as upregulation of autophagy proteins, microtubule-associated protein 1 light chain 3 (MAP-LC3) and autophagy gene 7 (ATG 7) in treated samples. AuNP treated cells also generated significantly more lipid hydroperoxides (p-value < 0.05), a positive indication of lipid peroxidation. Verification with western blot analysis for malondialdehyde (MDA) protein adducts confirmed the presence of oxidative damage. In addition, AuNP treatment also induced upregulation of antioxidants, stress response genes and protein expression. Exposure to AuNPs is a potential source of oxidative stress in human lung fibroblasts and autophagy may be a cellular defence mechanism against oxidative stress toxicity. © 2010 Elsevier Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2010.04.014
dc.sourceScopus
dc.subjectAutophagosomes
dc.subjectAutophagy
dc.subjectGold nanoparticles
dc.subjectLung fibroblasts
dc.subjectOxidative stress
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.contributor.departmentANATOMY
dc.contributor.departmentEPIDEMIOLOGY & PUBLIC HEALTH
dc.description.doi10.1016/j.biomaterials.2010.04.014
dc.description.sourcetitleBiomaterials
dc.description.volume31
dc.description.issue23
dc.description.page5996-6003
dc.identifier.isiut000279719600010
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