Please use this identifier to cite or link to this item: https://doi.org/10.1089/ten.tec.2013.0733
Title: Novel Use for Polyvinylpyrrolidone as a Macromolecular Crowder for Enhanced Extracellular Matrix Deposition and Cell Proliferation
Authors: Rashid, Rafi 
Lim, Natalie Sheng Jie 
Chee, Stella Min Ling
Png, Si Ning 
Wohland, Thorsten 
Raghunath, Michael 
Keywords: Science & Technology
Life Sciences & Biomedicine
Technology
Cell & Tissue Engineering
Cell Biology
Engineering, Biomedical
Materials Science, Biomaterials
Engineering
Materials Science
IN-VITRO
Issue Date: 1-Dec-2014
Publisher: MARY ANN LIEBERT, INC
Citation: Rashid, Rafi, Lim, Natalie Sheng Jie, Chee, Stella Min Ling, Png, Si Ning, Wohland, Thorsten, Raghunath, Michael (2014-12-01). Novel Use for Polyvinylpyrrolidone as a Macromolecular Crowder for Enhanced Extracellular Matrix Deposition and Cell Proliferation. TISSUE ENGINEERING PART C-METHODS 20 (12) : 994-1002. ScholarBank@NUS Repository. https://doi.org/10.1089/ten.tec.2013.0733
Abstract: Macromolecular crowding (MMC) is a biophysical effect that governs biochemical processes inside and outside of cells. Since standard cell culture media lack this effect, the physiological performance of differentiated and progenitor cells, including extracellular matrix (ECM) deposition, is impaired in vitro. To bring back physiological crowdedness to in vitro systems, we have previously introduced carbohydrate-based macromolecules to culture media and have achieved marked improvements with mixed MMC in terms of ECM deposition and differentiation of mesenchymal stem cells (MSCs). We show here that although this system is successful, it is limited, due to viscosity, to only 33% of the fractional volume occupancy (FVO) of full serum, which we calculated to have an FVO of approximately 54% v/v. We show here that full-serum FVO can be achieved using polyvinylpyrrolidone (PVP) 360kDa. Under these conditions, ECM deposition in human fibroblasts and MSCs is on par, if not stronger than, with original MMC protocols using carbohydrates, but with a viscosity that is not significantly changed. In addition, we have found that the proliferation rate for bone marrow-derived MSCs and fibroblasts increases slightly in the presence of PVP360, similar to that observed with carbohydrate-based crowders. A palette of MMC compounds is now emerging that enables us to tune the crowdedness of culture media seamlessly from interstitial fluid (9% FVO), in which the majority of tissue cells might be based, to serum environments mimicking intravascular conditions. Despite identical FVO's, individual crowder size effects play a role and different cell types appear to have preferences in terms of FVO and the crowder that this is achieved with. However, in the quest of crowders that we have predicted to have a smoother regulatory approval path, PVP is a highly interesting compound, as it has been widely used in the medical and food industries and shows a novel promising use in cell culture and tissue engineering.
Source Title: TISSUE ENGINEERING PART C-METHODS
URI: https://scholarbank.nus.edu.sg/handle/10635/243165
ISSN: 1937-3384
1937-3392
DOI: 10.1089/ten.tec.2013.0733
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
ten.tec.2013.0733.pdfPublished version522.53 kBAdobe PDF

OPEN

PublishedView/Download

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.