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Title: Determinants of Bioartificial Myocardial Graft Survival and Engraftment In Vivo
Authors: Mueller-Stahl, K.
Kofidis, T. 
Akhyari, P.
Lenz, A.
Haverich, A.
Lee, D.H.L.
Martinez, E.C. 
Woitek, F.
Issue Date: 2008
Citation: Mueller-Stahl, K., Kofidis, T., Akhyari, P., Lenz, A., Haverich, A., Lee, D.H.L., Martinez, E.C., Woitek, F. (2008). Determinants of Bioartificial Myocardial Graft Survival and Engraftment In Vivo. Journal of Heart and Lung Transplantation 27 (11) : 1242-1250. ScholarBank@NUS Repository.
Abstract: Background: The specific interactions between tissue-engineered grafts and host tissue are frequently neglected. The aim of this study was to describe and quantify the fate of a tissue-engineered cardiac graft in vivo. Methods: Neonatal rat cardiomyocytes were cast into a collagen mesh, forming a bioartificial myocardial tissue (AMT). After 7 days in vitro, four groups were formed (Group A: sham; Group B: matrix; Group C: AMT [with additional host treatment with cyclosporine and prednisolone]; Group D: AMT; each n = 5) and the tissue grafts were implanted into the muscle pouch of adult rats at 14, 21 and 28 days. Implants were stained for troponin-T, BrdU, MF-20, desmin, vimentin, Flk-1, CD8, CD4, pentachrome, PSR and H&E. Results: AMT cell count, cell proportion, contractility, viability and metabolism proved stable in vitro. Grafted cells decreased over time and were detected in Group C until the end of the experiment (Day 28), and in Group D until Day 21. Angiogenesis began at the peripheries and slowly progressed toward the cores of the grafts. The thickness and collagen content of the matrix remained stable in Group C for 14 days, and decreased in all groups until Day 28 (thickness: Group B, -66%; Group C, -50%; Group D, -100%). Grafts were predominately infiltrated by macrophages and stromal cells, and less so by lymphocytes (Group D > B > C). Conclusion: The differentiation of cardiac and non-cardiac grafted cells, infiltrating cells, scaffold kinetics and angiogenesis showed host immune responses and degree of angiogenesis to be the determinants for AMT graft survival. © 2008 International Society for Heart and Lung Transplantation.
Source Title: Journal of Heart and Lung Transplantation
ISSN: 10532498
DOI: 10.1016/j.healun.2008.08.003
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