Please use this identifier to cite or link to this item:
|Title:||Determinants of Bioartificial Myocardial Graft Survival and Engraftment In Vivo|
|Source:||Mueller-Stahl, K., Kofidis, T., Akhyari, P., Lenz, A., Haverich, A., Lee, D.H.L., Martinez, E.C., Woitek, F. (2008). Determinants of Bioartificial Myocardial Graft Survival and Engraftment In Vivo. Journal of Heart and Lung Transplantation 27 (11) : 1242-1250. ScholarBank@NUS Repository. https://doi.org/10.1016/j.healun.2008.08.003|
|Abstract:||Background: The specific interactions between tissue-engineered grafts and host tissue are frequently neglected. The aim of this study was to describe and quantify the fate of a tissue-engineered cardiac graft in vivo. Methods: Neonatal rat cardiomyocytes were cast into a collagen mesh, forming a bioartificial myocardial tissue (AMT). After 7 days in vitro, four groups were formed (Group A: sham; Group B: matrix; Group C: AMT [with additional host treatment with cyclosporine and prednisolone]; Group D: AMT; each n = 5) and the tissue grafts were implanted into the muscle pouch of adult rats at 14, 21 and 28 days. Implants were stained for troponin-T, BrdU, MF-20, desmin, vimentin, Flk-1, CD8, CD4, pentachrome, PSR and H&E. Results: AMT cell count, cell proportion, contractility, viability and metabolism proved stable in vitro. Grafted cells decreased over time and were detected in Group C until the end of the experiment (Day 28), and in Group D until Day 21. Angiogenesis began at the peripheries and slowly progressed toward the cores of the grafts. The thickness and collagen content of the matrix remained stable in Group C for 14 days, and decreased in all groups until Day 28 (thickness: Group B, -66%; Group C, -50%; Group D, -100%). Grafts were predominately infiltrated by macrophages and stromal cells, and less so by lymphocytes (Group D > B > C). Conclusion: The differentiation of cardiac and non-cardiac grafted cells, infiltrating cells, scaffold kinetics and angiogenesis showed host immune responses and degree of angiogenesis to be the determinants for AMT graft survival. © 2008 International Society for Heart and Lung Transplantation.|
|Source Title:||Journal of Heart and Lung Transplantation|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 27, 2018
WEB OF SCIENCETM
checked on Feb 27, 2018
checked on Feb 25, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.