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Title: 3D bioprinting and microscale organization of vascularized tissue constructs using collagen-based bioink
Authors: Muthusamy Senthilkumar 
Sathya Kannan
Marcus Lee
Vijayavenkataraman Sanjairaj
Wen-Feng Lu 
Ying Hsi,Jerry Fuh 
Sriram Gopu 
Tong Cao 
Keywords: additive manufacturing
stem cells
tissue engineering
vascularized tissue
Issue Date: 16-Jul-2021
Publisher: Wiley Periodicals LLC
Citation: Muthusamy Senthilkumar, Sathya Kannan, Marcus Lee, Vijayavenkataraman Sanjairaj, Wen-Feng Lu, Ying Hsi,Jerry Fuh, Sriram Gopu, Tong Cao (2021-07-16). 3D bioprinting and microscale organization of vascularized tissue constructs using collagen-based bioink. Biotechnology & Bioengineering 118 (8) : 3150 - 3163. ScholarBank@NUS Repository.
Rights: CC0 1.0 Universal
Abstract: Bioprinting three-dimensional (3D) tissue equivalents have progressed tremendously over the last decade. 3D bioprinting is currently being employed to develop larger and more physiologic tissues, and it is of particular interest to generate vasculature in biofabricated tissues to aid better perfusion and transport of nutrition. Having an advantage over manual culture systems by bringing together biological scaffold materials and cells in precise 3D spatial orientation, bioprinting could assist in placing endothelial cells in specific spatial locations within a 3D matrix to promote vessel formation at these predefined areas. Hence, in the present study, we investigated the use of bioprinting to generate tissue-level capillary-like networks in biofabricated tissue constructs. First, we developed a bioink using collagen type-1 supplemented with xanthan gum (XG) as a thickening agent. Using a commercial extrusion-based multi-head bioprinter and collagen-XG bioink, the component cells were spatially assembled, wherein the endothelial cells were bioprinted in a lattice pattern and sandwiched between bioprinted fibroblasts layers. 3D bioprinted constructs thus generated were stable, and maintained structural shape and form. Post-print culture of the bioprinted tissues resulted in endothelial sprouting and formation of interconnected capillary-like networks within the lattice pattern and between the fibroblast layers. Bioprinter-assisted spatial placement of endothelial cells resulted in fabrication of patterned prevascularized constructs that enable potential regenerative applications in the future.
Source Title: Biotechnology & Bioengineering
ISSN: 0006-3592
DOI: 10.1002/bit.27838
Rights: CC0 1.0 Universal
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