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Title: Domain-specific p53 mutants activate EGFR by distinct mechanisms exposing tissue-independent therapeutic vulnerabilities
Authors: Ho, Teresa Lai Fong 
Lee, May Yin
Goh, Hui Chin 
Ng, Germaine Yi Ning
Lee, Jane Jia Hui
Kannan, Srinivasaraghavan
Lim, Yan Ting 
Zhao, Tianyun 
Lim, Edwin Kok Hao
Phua, Cheryl Zi Jin
Lee, Yi Fei
Lim, Rebecca Yi Xuan
Ng, Perry Jun Hao
Yuan, Ju
Chan, Dedrick Kok Hong 
Lieske, Bettina 
Chong, Choon Seng 
Lee, Kuok Chung
Lum, Jeffrey 
Cheong, Wai Kit
Yeoh, Khay Guan 
Tan, Ker Kan 
Sobota, Radoslaw M
Verma, Chandra S 
Lane, David P
Tam, Wai Leong 
Venkitaraman, Ashok R 
Issue Date: 28-Mar-2023
Publisher: Springer Science and Business Media LLC
Citation: Ho, Teresa Lai Fong, Lee, May Yin, Goh, Hui Chin, Ng, Germaine Yi Ning, Lee, Jane Jia Hui, Kannan, Srinivasaraghavan, Lim, Yan Ting, Zhao, Tianyun, Lim, Edwin Kok Hao, Phua, Cheryl Zi Jin, Lee, Yi Fei, Lim, Rebecca Yi Xuan, Ng, Perry Jun Hao, Yuan, Ju, Chan, Dedrick Kok Hong, Lieske, Bettina, Chong, Choon Seng, Lee, Kuok Chung, Lum, Jeffrey, Cheong, Wai Kit, Yeoh, Khay Guan, Tan, Ker Kan, Sobota, Radoslaw M, Verma, Chandra S, Lane, David P, Tam, Wai Leong, Venkitaraman, Ashok R (2023-03-28). Domain-specific p53 mutants activate EGFR by distinct mechanisms exposing tissue-independent therapeutic vulnerabilities. Nature Communications 14 (1). ScholarBank@NUS Repository.
Abstract: AbstractMis-sense mutations affecting TP53 promote carcinogenesis both by inactivating tumor suppression, and by conferring pro-carcinogenic activities. We report here that p53 DNA-binding domain (DBD) and transactivation domain (TAD) mis-sense mutants unexpectedly activate pro-carcinogenic epidermal growth factor receptor (EGFR) signaling via distinct, previously unrecognized molecular mechanisms. DBD- and TAD-specific TP53 mutants exhibited different cellular localization and induced distinct gene expression profiles. In multiple tissues, EGFR is stabilized by TAD and DBD mutants in the cytosolic and nuclear compartments respectively. TAD mutants promote EGFR-mediated signaling by enhancing EGFR interaction with AKT via DDX31 in the cytosol. Conversely, DBD mutants maintain EGFR activity in the nucleus, by blocking EGFR interaction with the phosphatase SHP1, triggering c-Myc and Cyclin D1 upregulation. Our findings suggest that p53 mutants carrying gain-of-function, mis-sense mutations affecting two different domains form new protein complexes that promote carcinogenesis by enhancing EGFR signaling via distinctive mechanisms, exposing clinically relevant therapeutic vulnerabilities.
Source Title: Nature Communications
ISSN: 2041-1723
DOI: 10.1038/s41467-023-37223-3
Appears in Collections:Staff Publications

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