Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/234941
Title: Natural IgG antibodies provide innate protection against ficolin-opsonized bacteria
Authors: Panda, Saswati 
Zhang, Jing
Tan, Nguan Soon 
Ho, Bow 
Ding, Jeak Ling 
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Cell Biology
bacterial recognition
IgG:lectin immune complexes
infection-inflammation condition
lectins
natural antibodies
C-REACTIVE PROTEIN
CYTIDINE DEAMINASE AID
B-CELLS
COMPLEMENT ACTIVATION
DENDRITIC CELLS
RECOGNITION
HYPERMUTATION
ANTIGEN
LECTIN
MACROPHAGES
Issue Date: 13-Nov-2013
Publisher: WILEY
Citation: Panda, Saswati, Zhang, Jing, Tan, Nguan Soon, Ho, Bow, Ding, Jeak Ling (2013-11-13). Natural IgG antibodies provide innate protection against ficolin-opsonized bacteria. EMBO JOURNAL 32 (22) : 2905-2919. ScholarBank@NUS Repository.
Abstract: For nearly five decades since its discovery, the role of natural IgG, which pre-exists in neonates and uninfected individuals, has remained unclear due to the general perception that natural antibodies lack affinity for pathogens. Here, we show for the first time that natural IgG recognizes a spectrum of bacteria through lectins like ficolin and mannose binding lectin (MBL). Infection-inflammation condition markedly increased the affinity of natural IgG for bacteria associated with ficolins. After opsonization with IgG:ficolin complex, the bacteria were phagocytosed by monocytes via FcγRI. Infection of C3 -/- mice indicated that the natural IgG-mediated immune complex was formed independently of C3. AID -/- mice lacking IgG were susceptible to infection, unless reconstituted with natural IgG. Thus, we have proven that natural IgG is not quiescent; rather, it plays a vital and immediate role in immune defense. Our findings provide a fresh perspective on natural antibodies, opening new avenues to explore host-microbe interaction. © 2013 European Molecular Biology Organization.
Source Title: EMBO JOURNAL
URI: https://scholarbank.nus.edu.sg/handle/10635/234941
ISSN: 0261-4189
1460-2075
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