Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.nano.2019.02.024
Title: Surface protein engineering increases the circulation time of a cell membrane-based nanotherapeutic
Authors: Krishnamurthy, Sangeetha 
Muthukumaran, Padmalosini
Jayakumar, Muthu Kumara Gnanasammandhan 
Lisse, Domenik
Masurkar, Nihar D
Xu, Chenjie
Chan, Juliana M 
Drum, Chester L 
Keywords: Science & Technology
Life Sciences & Biomedicine
Nanoscience & Nanotechnology
Medicine, Research & Experimental
Science & Technology - Other Topics
Research & Experimental Medicine
PASylation
Nanoghosts
Cell ghosts
Lipid polymer hybrid nanoparticles
Synthetic biology
Drug delivery
Nanomedicine
PLASMA HALF-LIFE
POLYETHYLENE-GLYCOL
PROSTATE-CANCER
NANOPARTICLES
DELIVERY
PEGYLATION
THERAPY
PASYLATION
CARRIER
Issue Date: 1-Jun-2019
Publisher: ELSEVIER SCIENCE BV
Citation: Krishnamurthy, Sangeetha, Muthukumaran, Padmalosini, Jayakumar, Muthu Kumara Gnanasammandhan, Lisse, Domenik, Masurkar, Nihar D, Xu, Chenjie, Chan, Juliana M, Drum, Chester L (2019-06-01). Surface protein engineering increases the circulation time of a cell membrane-based nanotherapeutic. NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE 18 : 169-178. ScholarBank@NUS Repository. https://doi.org/10.1016/j.nano.2019.02.024
Abstract: Mammalian cell membranes are often incompatible with chemical modifications typically used to increase circulation half-life. Using cellular nanoghosts as a model, we show that proline-alanine-serine (PAS) peptide sequences expressed on the membrane surface can extend the circulation time of a cell membrane derived nanotherapeutic. Membrane expression of a PAS 40 repeat sequence decreased protein binding and resulted in a 90% decrease in macrophage uptake when compared with non-PASylated controls (P ≤ 0.05). PASylation also extended circulation half-life (t 1/2 = 37 h) compared with non-PASylated controls (t 1/2 = 10.5 h) (P ≤ 0.005), resulting in ~7-fold higher in vivo serum concentrations at 24 h and 48 h (P ≤ 0.005). Genetically engineered membrane expression of PAS repeats may offer an alternative to PEGylation and provide extended circulation times for cellular membrane-derived nanotherapeutics.
Source Title: NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
URI: https://scholarbank.nus.edu.sg/handle/10635/234694
ISSN: 15499634
15499642
DOI: 10.1016/j.nano.2019.02.024
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