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Title: Loss of YhcB results in dysregulation of coordinated peptidoglycan, LPS and phospholipid synthesis during Escherichia coli cell growth
Authors: Goodall, Emily C. A.
Isom, Georgia L.
Rooke, Jessica L.
Pullela, Karthik
Icke, Christopher
Yang, Zihao
Boelter, Gabriela
Jones, Alun
Warner, Isabel
Da Costa, Rochelle
Zhang, Bing
Rae, James
Tan, Wee Boon 
Winkle, Matthias
Delhaye, Antoine
Heinz, Eva
Collet, Jean-Francois
Cunningham, Adam F.
Blaskovich, Mark A.
Parton, Robert G.
Cole, Jeff A.
Banzhaf, Manuel
Chng, Shu-Sin 
Vollmer, Waldemar
Bryant, Jack A.
Henderson, Ian R.
Issue Date: 23-Dec-2021
Publisher: Public Library of Science
Citation: Goodall, Emily C. A., Isom, Georgia L., Rooke, Jessica L., Pullela, Karthik, Icke, Christopher, Yang, Zihao, Boelter, Gabriela, Jones, Alun, Warner, Isabel, Da Costa, Rochelle, Zhang, Bing, Rae, James, Tan, Wee Boon, Winkle, Matthias, Delhaye, Antoine, Heinz, Eva, Collet, Jean-Francois, Cunningham, Adam F., Blaskovich, Mark A., Parton, Robert G., Cole, Jeff A., Banzhaf, Manuel, Chng, Shu-Sin, Vollmer, Waldemar, Bryant, Jack A., Henderson, Ian R. (2021-12-23). Loss of YhcB results in dysregulation of coordinated peptidoglycan, LPS and phospholipid synthesis during Escherichia coli cell growth. PLoS Genetics 17 (12) : e1009586. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: The cell envelope is essential for viability in all domains of life. It retains enzymes and substrates within a confined space while providing a protective barrier to the external environment. Destabilising the envelope of bacterial pathogens is a common strategy employed by antimicrobial treatment. However, even in one of the best studied organisms, Escherichia coli, there remain gaps in our understanding of how the synthesis of the successive layers of the cell envelope are coordinated during growth and cell division. Here, we used a whole-genome phenotypic screen to identify mutants with a defective cell envelope. We report that loss of yhcB, a conserved gene of unknown function, results in loss of envelope stability, increased cell permeability and dysregulated control of cell size. Using whole genome transposon mutagenesis strategies, we report the comprehensive genetic interaction network of yhcB, revealing all genes with a synthetic negative and a synthetic positive relationship. These genes include those previously reported to have a role in cell envelope biogenesis. Surprisingly, we identified genes previously annotated as essential that became non-essential in a ?yhcB background. Subsequent analyses suggest that YhcB functions at the junction of several envelope biosynthetic pathways coordinating the spatiotemporal growth of the cell, highlighting YhcB as an as yet unexplored antimicrobial target. Copyright: © 2021 Goodall et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Source Title: PLoS Genetics
ISSN: 1553-7390
DOI: 10.1371/journal.pgen.1009586
Rights: Attribution 4.0 International
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