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Title: Anti-fungal hevein-like peptides biosynthesized from quinoa cleavable hololectins
Authors: Loo, Shining
Tay, Stephanie, V
Kam, Antony
Tang, Fan
Fan, Jing-Song 
Yang, Daiwen 
Tam, James P.
Keywords: Anti-fungal
hevein-like peptide
Issue Date: 29-Sep-2021
Publisher: MDPI
Citation: Loo, Shining, Tay, Stephanie, V, Kam, Antony, Tang, Fan, Fan, Jing-Song, Yang, Daiwen, Tam, James P. (2021-09-29). Anti-fungal hevein-like peptides biosynthesized from quinoa cleavable hololectins. Molecules 26 (19) : 5909. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Chitin-binding hevein-like peptides (CB-HLPs) belong to a family of cysteine-rich peptides that play important roles in plant stress and defense mechanisms. CB-HLPs are ribosomally synthesized peptides that are known to be bioprocessed from the following two types of three-domain CB-HLP precursor architectures: cargo-carrying and non-cargo-carrying. Here, we report the identification and characterization of chenotides biosynthesized from the third type of precursors, which are cleavable hololectins of the quinoa (Chenopodium quinoa) family. Chenotides are 6-Cys-CB-HLPs of 29–31 amino acids, which have a third type of precursor architecture that encompasses a canonical chitin-binding domain that is involved in chitin binding and anti-fungal activities. Microbroth dilution assays and microscopic analyses showed that chenotides are effective against phyto-pathogenic fungi in the micromolar range. Structure determination revealed that chenotides are cystine knot-ted and highly compact, which could confer resistance against heat and proteolytic degradation. Importantly, chenotides are connected by a novel 18-residue Gly/Ala-rich linker that is a target for bioprocessing by cathepsin-like endopeptidases. Taken together, our findings reveal that chenotides are a new family of CB-HLPs from quinoa that are synthesized as a single multi-modular unit and bioprocessed to yield individual mature CB-HLPs. Importantly, such precursors constitute a new family of cleavable hololectins. This unusual feature could increase the biosynthetic efficiency of anti-fungal CB-HLPs, to provide an evolutionary advantage for plant survival and reproduction. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: Molecules
ISSN: 1420-3049
DOI: 10.3390/molecules26195909
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

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