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Title: Zymography for picogram detection of lipase and esterase activities
Authors: Ng, Andre Mong Jie 
Zhang, Hongfang
Giang Kien Truc Nguyen
Keywords: Esterase
Issue Date: 11-Mar-2021
Publisher: MDPI AG
Citation: Ng, Andre Mong Jie, Zhang, Hongfang, Giang Kien Truc Nguyen (2021-03-11). Zymography for picogram detection of lipase and esterase activities. Molecules 26 (6) : 1542. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Lipases and esterases are important catalysts with wide varieties of industrial applications. Although many methods have been established for detecting their activities, a simple and sensitive approach for picogram detection of lipolytic enzyme quantity is still highly desirable. Here we report a lipase detection assay which is 1000-fold more sensitive than previously reported methods. Our assay enables the detection of as low as 5 pg and 180 pg of lipolytic activity by direct spotting and zymography, respectively. Furthermore, we demonstrated that the detection sensitivity was adjustable by varying the buffering capacity, which allows for screening of both high and low abundance lipolytic enzymes. Coupled with liquid chromatography-mass spectrometry, our method provides a useful tool for sensitive detection and identification of lipolytic enzymes. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: Molecules
ISSN: 1420-3049
DOI: 10.3390/molecules26061542
Rights: Attribution 4.0 International
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