Please use this identifier to cite or link to this item: https://doi.org/10.3390/molecules26061542
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dc.titleZymography for picogram detection of lipase and esterase activities
dc.contributor.authorNg, Andre Mong Jie
dc.contributor.authorZhang, Hongfang
dc.contributor.authorGiang Kien Truc Nguyen
dc.date.accessioned2022-10-13T01:13:10Z
dc.date.available2022-10-13T01:13:10Z
dc.date.issued2021-03-11
dc.identifier.citationNg, Andre Mong Jie, Zhang, Hongfang, Giang Kien Truc Nguyen (2021-03-11). Zymography for picogram detection of lipase and esterase activities. Molecules 26 (6) : 1542. ScholarBank@NUS Repository. https://doi.org/10.3390/molecules26061542
dc.identifier.issn1420-3049
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/232821
dc.description.abstractLipases and esterases are important catalysts with wide varieties of industrial applications. Although many methods have been established for detecting their activities, a simple and sensitive approach for picogram detection of lipolytic enzyme quantity is still highly desirable. Here we report a lipase detection assay which is 1000-fold more sensitive than previously reported methods. Our assay enables the detection of as low as 5 pg and 180 pg of lipolytic activity by direct spotting and zymography, respectively. Furthermore, we demonstrated that the detection sensitivity was adjustable by varying the buffering capacity, which allows for screening of both high and low abundance lipolytic enzymes. Coupled with liquid chromatography-mass spectrometry, our method provides a useful tool for sensitive detection and identification of lipolytic enzymes. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
dc.publisherMDPI AG
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2021
dc.subjectEsterase
dc.subjectLipase
dc.subjectPicogram
dc.subjectZymogram
dc.typeArticle
dc.contributor.departmentLIFE SCIENCES INSTITUTE
dc.description.doi10.3390/molecules26061542
dc.description.sourcetitleMolecules
dc.description.volume26
dc.description.issue6
dc.description.page1542
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