Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.syapm.2021.126223
Title: Proposal of Carbonactinosporaceae fam. nov. within the class Actinomycetia. Reclassification of Streptomyces thermoautotrophicus as Carbonactinospora thermoautotrophica gen. nov., comb. nov
Authors: Volpiano, Camila Gazolla
Sant'Anna, Fernando Hayashi
da Mota, Fabio Faria
Sangal, Vartul
Sutcliffe, Iain
Munusamy, Madhaiyan
Saravanan, Venkatakrishnan Sivaraj
See-Too, Wah-Seng
Pereira Passaglia, Luciane Maria
Rosado, Alexandre Soares
Keywords: Actinobacteria
Chemoautotrophic bacteria
Genomics metrics
Phylogenetic systematics
Issue Date: 1-Jul-2021
Publisher: Elsevier GmbH
Citation: Volpiano, Camila Gazolla, Sant'Anna, Fernando Hayashi, da Mota, Fabio Faria, Sangal, Vartul, Sutcliffe, Iain, Munusamy, Madhaiyan, Saravanan, Venkatakrishnan Sivaraj, See-Too, Wah-Seng, Pereira Passaglia, Luciane Maria, Rosado, Alexandre Soares (2021-07-01). Proposal of Carbonactinosporaceae fam. nov. within the class Actinomycetia. Reclassification of Streptomyces thermoautotrophicus as Carbonactinospora thermoautotrophica gen. nov., comb. nov. Systematic and Applied Microbiology 44 (4) : 126223. ScholarBank@NUS Repository. https://doi.org/10.1016/j.syapm.2021.126223
Rights: Attribution 4.0 International
Abstract: Streptomyces thermoautotrophicus UBT1T has been suggested to merit generic status due to its phylogenetic placement and distinctive phenotypes among Actinomycetia. To evaluate whether ‘S. thermoautotrophicus’ represents a higher taxonomic rank, ‘S. thermoautotrophicus’ strains UBT1T and H1 were compared to Actinomycetia using 16S rRNA gene sequences and comparative genome analyses. The UBT1T and H1 genomes each contain at least two different 16S rRNA sequences, which are closely related to those of Acidothermus cellulolyticus (order Acidothermales). In multigene-based phylogenomic trees, UBT1T and H1 typically formed a sister group to the Streptosporangiales-Acidothermales clade. The Average Amino Acid Identity, Percentage of Conserved Proteins, and whole-genome Average Nucleotide Identity (Alignment Fraction) values were ≤58.5%, ≤48%, ≤75.5% (0.3) between ‘S. thermoautotrophicus’ and Streptosporangiales members, all below the respective thresholds for delineating genera. The values for genomics comparisons between strains UBT1T and H1 with Acidothermales, as well as members of the genus Streptomyces, were even lower. A review of the ‘S. thermoautotrophicus’ proteomic profiles and KEGG orthology demonstrated that UBT1T and H1 present pronounced differences, both tested and predicted, in phenotypic and chemotaxonomic characteristics compared to its sister clades and Streptomyces. The distinct phylogenetic position and the combination of genotypic and phenotypic characteristics justify the proposal of Carbonactinospora gen. nov., with the type species Carbonactinospora thermoautotrophica comb. nov. (type strain UBT1T, = DSM 100163T = KCTC 49540T) belonging to Carbonactinosporaceae fam. nov. within Actinomycetia. © 2021 The Author(s)
Source Title: Systematic and Applied Microbiology
URI: https://scholarbank.nus.edu.sg/handle/10635/232222
ISSN: 0723-2020
DOI: 10.1016/j.syapm.2021.126223
Rights: Attribution 4.0 International
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