Please use this identifier to cite or link to this item:
Title: Engineered ns1 for sensitive, specifi c zika virus diagnosis from patient serology
Authors: Yap, Thai Leong
Hong, Shin Yee
Soh, Jun Hui
Ravichandraprabhu, Lekha
Lim, Vanessa W. X.
Chan, Hsi-Min
Ong, Tommy Z. X.
Chua, Ying Ping
Koh, Shi En
Wang, Huajing
Leo, Yee Sin 
Ying, Jackie Y.
Sun, William 
Issue Date: 1-May-2021
Publisher: Centers for Disease Control and Prevention (CDC)
Citation: Yap, Thai Leong, Hong, Shin Yee, Soh, Jun Hui, Ravichandraprabhu, Lekha, Lim, Vanessa W. X., Chan, Hsi-Min, Ong, Tommy Z. X., Chua, Ying Ping, Koh, Shi En, Wang, Huajing, Leo, Yee Sin, Ying, Jackie Y., Sun, William (2021-05-01). Engineered ns1 for sensitive, specifi c zika virus diagnosis from patient serology. Emerging Infectious Diseases 27 (5) : 1427-1437. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Dengue virus (DENV) and Zika virus (ZIKV) belong to the Flaviviridae family of viruses spread by Aedes aegypti mosquitoes in tropical and subtropical areas. Accurate diagnostic tests to diff erentiate the 2 infections are necessary for patient management and disease control. Using characterized ZIKV and DENV patient plasma in a blind manner, we validated an ELISA and a rapid immunochromatographic test for ZIKV detection. We engineered the ZIKV nonstructural protein 1 (NS1) for sensitive serologic detection with low cross reactivity against dengue and developed monoclonal antibodies specifi c for the ZIKV NS1 antigen. As expected, the serologic assays performed better with convalescent than acute plasma samples; the sensitivity ranged from 71% to 88%, depending on the performance of individual tests (IgM/ IgG/NS1). Although serologic tests were generally less sensitive with acute samples, our ZIKV NS1 antibodies were able to complement the serologic tests to achieve greater sensitivity for detecting early infections. © 2021 Centers for Disease Control and Prevention (CDC). All rights reserved.
Source Title: Emerging Infectious Diseases
ISSN: 1080-6040
DOI: 10.3201/eid2705.190121
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_3201_eid2705_190121.pdf1.97 MBAdobe PDF



Google ScholarTM



This item is licensed under a Creative Commons License Creative Commons