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Title: Enhancer-derived long non-coding RNAs CCAT1 and CCAT2 at rs6983267 has limited predictability for early stage colorectal carcinoma metastasis
Authors: Thean, Lai Fun
Blöcker, Christopher
Li, Hui Hua
Lo, Michelle
Wong, Michelle
Tang, Choong Leong
Tan, Emile K. W.
Rozen, Steven G. 
Cheah, Peh Yean 
Issue Date: 11-Jan-2021
Publisher: Nature Research
Citation: Thean, Lai Fun, Blöcker, Christopher, Li, Hui Hua, Lo, Michelle, Wong, Michelle, Tang, Choong Leong, Tan, Emile K. W., Rozen, Steven G., Cheah, Peh Yean (2021-01-11). Enhancer-derived long non-coding RNAs CCAT1 and CCAT2 at rs6983267 has limited predictability for early stage colorectal carcinoma metastasis. Scientific Reports 11 (1) : 404. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Up-regulation of long non-coding RNAs (lncRNAs), colon-cancer associated transcript (CCAT) 1 and 2, was associated with worse prognosis in colorectal cancer (CRC). Nevertheless, their role in predicting metastasis in early-stage CRC is unclear. We measured the expression of CCAT1, CCAT2 and their oncotarget, c-Myc, in 150 matched mucosa-tumour samples of early-stage microsatellite-stable Chinese CRC patients with definitive metastasis status by multiplex real-time RT-PCR assay. Expression of CCAT1, CCAT2 and c-Myc were significantly up-regulated in the tumours compared to matched mucosa (p < 0.0001). The expression of c-Myc in the tumours was significantly correlated to time to metastasis [hazard ratio = 1.47 (1.10–1.97)] and the risk genotype (GG) of rs6983267, located within CCAT2. Expression of c-Myc and CCAT2 in the tumour were also significantly up-regulated in metastasis-positive compared to metastasis-negative patients (p = 0.009 and p = 0.04 respectively). Nevertheless, integrating the expression of CCAT1 and CCAT2 by the Random Forest classifier did not improve the predictive values of ColoMet19, the mRNA-based predictor for metastasis previously developed on the same series of tumours. The role of these two lncRNAs is probably mitigated via their oncotarget, c-Myc, which was not ranked high enough previously to be included in ColoMet19. © 2021, The Author(s).
Source Title: Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/s41598-020-79906-7
Rights: Attribution 4.0 International
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