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https://doi.org/10.1016/j.actbio.2020.10.039
Title: | Directionalities of magnetic fields and topographic scaffolds synergise to enhance MSC chondrogenesis | Authors: | Celik, Cenk Franco-Obregon, Alfredo Lee, Eng Hin Hui, James HP Yang, Zheng |
Keywords: | Science & Technology Technology Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Pulsed electromagnetic fields mesenchymal stem cells electrospun fibres chondrogenesis mechanotransduction MESENCHYMAL STEM-CELLS FOCAL ADHESION KINASE FREQUENCY ELECTROMAGNETIC-FIELD GENE-EXPRESSION DIFFERENTIATION PHENOTYPE YAP/TAZ CYTOSKELETON STIFFNESS HYPERTROPHY |
Issue Date: | 1-Jan-2021 | Publisher: | ELSEVIER SCI LTD | Citation: | Celik, Cenk, Franco-Obregon, Alfredo, Lee, Eng Hin, Hui, James HP, Yang, Zheng (2021-01-01). Directionalities of magnetic fields and topographic scaffolds synergise to enhance MSC chondrogenesis. ACTA BIOMATERIALIA 119 : 169-183. ScholarBank@NUS Repository. https://doi.org/10.1016/j.actbio.2020.10.039 | Abstract: | Mesenchymal stem cell (MSC) chondrogenesis is modulated by diverse biophysical cues. We have previously shown that brief, low-amplitude pulsed electromagnetic fields (PEMFs) differentially enhance MSC chondrogenesis in scaffold-free pellet cultures versus conventional tissue culture plastic (TCP), indicating an interplay between magnetism and micromechanical environment. Here, we examined the influence of PEMF directionality over the chondrogenic differentiation of MSCs laden on electrospun fibrous scaffolds of either random (RND) or aligned (ALN) orientations. Correlating MSCs’ chondrogenic outcome to pFAK activation and YAP localisation, MSCs on the RND scaffolds experienced the least amount of resting mechanical stress and underwent greatest chondrogenic differentiation in response to brief PEMF exposure (10 min at 1 mT) perpendicular to the dominant plane of the scaffolds (Z-directed). By contrast, in MSC-impregnated RND scaffolds, greatest mitochondrial respiration resulted from X-directed PEMF exposure (parallel to the scaffold plane), and was associated with curtailed chondrogenesis. MSCs on TCP or the ALN scaffolds exhibited greater resting mechanical stress and accordingly, were unresponsive, or negatively responsive, to PEMF exposure from all directions. The efficacy of PEMF-induced MSC chondrogenesis is hence regulated in a multifaceted manner involving focal adhesion dynamics, as well as mitochondrial responses, culminating in a final cellular response. The combined contributions of micromechanical environment and magnetic field orientation hence will need to be considered when designing magnetic exposure paradigms. | Source Title: | ACTA BIOMATERIALIA | URI: | https://scholarbank.nus.edu.sg/handle/10635/231337 | ISSN: | 1742-7061 1878-7568 |
DOI: | 10.1016/j.actbio.2020.10.039 |
Appears in Collections: | Staff Publications Elements |
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