Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.actbio.2020.10.039
Title: Directionalities of magnetic fields and topographic scaffolds synergise to enhance MSC chondrogenesis
Authors: Celik, Cenk
Franco-Obregon, Alfredo 
Lee, Eng Hin 
Hui, James HP
Yang, Zheng 
Keywords: Science & Technology
Technology
Engineering, Biomedical
Materials Science, Biomaterials
Engineering
Materials Science
Pulsed electromagnetic fields
mesenchymal stem cells
electrospun fibres
chondrogenesis
mechanotransduction
MESENCHYMAL STEM-CELLS
FOCAL ADHESION KINASE
FREQUENCY ELECTROMAGNETIC-FIELD
GENE-EXPRESSION
DIFFERENTIATION
PHENOTYPE
YAP/TAZ
CYTOSKELETON
STIFFNESS
HYPERTROPHY
Issue Date: 1-Jan-2021
Publisher: ELSEVIER SCI LTD
Citation: Celik, Cenk, Franco-Obregon, Alfredo, Lee, Eng Hin, Hui, James HP, Yang, Zheng (2021-01-01). Directionalities of magnetic fields and topographic scaffolds synergise to enhance MSC chondrogenesis. ACTA BIOMATERIALIA 119 : 169-183. ScholarBank@NUS Repository. https://doi.org/10.1016/j.actbio.2020.10.039
Abstract: Mesenchymal stem cell (MSC) chondrogenesis is modulated by diverse biophysical cues. We have previously shown that brief, low-amplitude pulsed electromagnetic fields (PEMFs) differentially enhance MSC chondrogenesis in scaffold-free pellet cultures versus conventional tissue culture plastic (TCP), indicating an interplay between magnetism and micromechanical environment. Here, we examined the influence of PEMF directionality over the chondrogenic differentiation of MSCs laden on electrospun fibrous scaffolds of either random (RND) or aligned (ALN) orientations. Correlating MSCs’ chondrogenic outcome to pFAK activation and YAP localisation, MSCs on the RND scaffolds experienced the least amount of resting mechanical stress and underwent greatest chondrogenic differentiation in response to brief PEMF exposure (10 min at 1 mT) perpendicular to the dominant plane of the scaffolds (Z-directed). By contrast, in MSC-impregnated RND scaffolds, greatest mitochondrial respiration resulted from X-directed PEMF exposure (parallel to the scaffold plane), and was associated with curtailed chondrogenesis. MSCs on TCP or the ALN scaffolds exhibited greater resting mechanical stress and accordingly, were unresponsive, or negatively responsive, to PEMF exposure from all directions. The efficacy of PEMF-induced MSC chondrogenesis is hence regulated in a multifaceted manner involving focal adhesion dynamics, as well as mitochondrial responses, culminating in a final cellular response. The combined contributions of micromechanical environment and magnetic field orientation hence will need to be considered when designing magnetic exposure paradigms.
Source Title: ACTA BIOMATERIALIA
URI: https://scholarbank.nus.edu.sg/handle/10635/231337
ISSN: 1742-7061
1878-7568
DOI: 10.1016/j.actbio.2020.10.039
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