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|Title:||Bacterial species associated with persistent apical periodontitis exert differential effects on osteogenic differentiation||Authors:||Chow A. T.
Quah S. Y.
Lim K. C.
Yu V. S. H.
Tan K. S.
Persistent Apical Periodontitis
|Issue Date:||15-Jan-2019||Publisher:||John Wiley & Sons Ltd||Citation:||Chow A. T., Quah S. Y., Bergenholtz G., Lim K. C., Yu V. S. H., Tan K. S. (2019-01-15). Bacterial species associated with persistent apical periodontitis exert differential effects on osteogenic differentiation. International Endodontic Journal 52 (2) : 201-210. ScholarBank@NUS Repository. https://doi.org/10.1111/iej.12994||Abstract:||Aim: To determine if bacteria associated with persistent apical periodontitis induce species-specific pro-inflammatory cytokine responses in macrophages, and the effects of this species-specific microenvironment on osteogenic differentiation. Methodology: Macrophages were exposed to Enterococcus faecalis, Streptococcus oralis, S. mitis, Fusobacterium nucleatum, Treponema denticola or Tannerella forsythia, and levels of TNF-α and IL-1β elicited were determined by immunoassay. Following treatment of MG-63 pre-osteoblasts with conditioned media from bacteria-exposed macrophages, osteogenic differentiation and viability of osteoblasts were analyzed by Alizarin Red Staining and MTS assay respectively. Statistical analysis was carried out by one-way ANOVA with the Tukey post-hoc test. Differences were considered to be significant if p<0.05. Results: Macrophages exposed to Gram-positive bacteria did not produce significant amounts of cytokines. F. nucleatum-challenged macrophages produced up to 4-fold more TNF-α and IL-1β compared to T. denticola or T. forsythia. Only conditioned media from macrophages treated with Gram-negative bacteria decreased mineralization and viability of osteoblasts. Conclusions: Gram-positive bacteria did not impact osteogenic differentiation and appeared innocuous. Gram-negative bacteria, in particular F. nucleatum elicited an enhanced pro-inflammatory response in macrophages, inhibited osteogenic differentiation and reduced cell viability. The findings suggest that the presence of this organism could potentially increase the severity of persistent apical periodontitis.||Source Title:||International Endodontic Journal||URI:||https://scholarbank.nus.edu.sg/handle/10635/231003||DOI:||10.1111/iej.12994|
|Appears in Collections:||Staff Publications|
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