Please use this identifier to cite or link to this item: https://doi.org/10.1111/iej.12994
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dc.titleBacterial species associated with persistent apical periodontitis exert differential effects on osteogenic differentiation
dc.contributor.authorChow A. T.
dc.contributor.authorQuah S. Y.
dc.contributor.authorBergenholtz G.
dc.contributor.authorLim K. C.
dc.contributor.authorYu V. S. H.
dc.contributor.authorTan K. S.
dc.date.accessioned2022-09-09T04:10:32Z
dc.date.available2022-09-09T04:10:32Z
dc.date.issued2019-01-15
dc.identifier.citationChow A. T., Quah S. Y., Bergenholtz G., Lim K. C., Yu V. S. H., Tan K. S. (2019-01-15). Bacterial species associated with persistent apical periodontitis exert differential effects on osteogenic differentiation. International Endodontic Journal 52 (2) : 201-210. ScholarBank@NUS Repository. https://doi.org/10.1111/iej.12994
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/231003
dc.description.abstractAim: To determine if bacteria associated with persistent apical periodontitis induce species-specific pro-inflammatory cytokine responses in macrophages, and the effects of this species-specific microenvironment on osteogenic differentiation. Methodology: Macrophages were exposed to Enterococcus faecalis, Streptococcus oralis, S. mitis, Fusobacterium nucleatum, Treponema denticola or Tannerella forsythia, and levels of TNF-α and IL-1β elicited were determined by immunoassay. Following treatment of MG-63 pre-osteoblasts with conditioned media from bacteria-exposed macrophages, osteogenic differentiation and viability of osteoblasts were analyzed by Alizarin Red Staining and MTS assay respectively. Statistical analysis was carried out by one-way ANOVA with the Tukey post-hoc test. Differences were considered to be significant if p<0.05. Results: Macrophages exposed to Gram-positive bacteria did not produce significant amounts of cytokines. F. nucleatum-challenged macrophages produced up to 4-fold more TNF-α and IL-1β compared to T. denticola or T. forsythia. Only conditioned media from macrophages treated with Gram-negative bacteria decreased mineralization and viability of osteoblasts. Conclusions: Gram-positive bacteria did not impact osteogenic differentiation and appeared innocuous. Gram-negative bacteria, in particular F. nucleatum elicited an enhanced pro-inflammatory response in macrophages, inhibited osteogenic differentiation and reduced cell viability. The findings suggest that the presence of this organism could potentially increase the severity of persistent apical periodontitis.
dc.description.urihttps://onlinelibrary.wiley.com/doi/10.1111/iej.12994
dc.language.isoen
dc.publisherJohn Wiley & Sons Ltd
dc.subjectFusobacterium nucleatum
dc.subjectInflammation
dc.subjectMacrophages
dc.subjectOsteoblasts
dc.subjectPersistent Apical Periodontitis
dc.typeArticle
dc.contributor.departmentDEAN'S OFFICE (DENTISTRY)
dc.contributor.departmentDEPT OF DENTISTRY
dc.description.doi10.1111/iej.12994
dc.description.sourcetitleInternational Endodontic Journal
dc.description.volume52
dc.description.issue2
dc.description.page201-210
dc.published.statePublished
dc.grant.fundingagencyNational University of Singapore
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