Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jchromb.2004.02.020
Title: Simple reversed-phase ion-pair liquid chromatography assay for the simultaneous determination of mycophenolic acid and its glucuronide metabolite in human plasma and urine
Authors: Yau, WP 
Vathsala, A 
Lou, HX
Chan, E 
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Chemistry, Analytical
Biochemistry & Molecular Biology
Chemistry
mycophenolic acid
mycophenolic acid glucuronide
SIMPLE HPLC METHOD
DIFFERENT DOSAGES
MOFETIL
PHARMACOKINETICS
CONJUGATE
EFFICACY
SAFETY
Issue Date: 5-Jun-2004
Publisher: ELSEVIER
Citation: Yau, WP, Vathsala, A, Lou, HX, Chan, E (2004-06-05). Simple reversed-phase ion-pair liquid chromatography assay for the simultaneous determination of mycophenolic acid and its glucuronide metabolite in human plasma and urine. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 805 (1) : 101-112. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2004.02.020
Abstract: A simple and reproducible reversed-phase ion-pair high-performance liquid chromatographic (HPLC) method using isocratic elution with UV absorbance detection is presented for the simultaneous quantitation of mycophenolic acid (MPA) and MPA-glucuronide (MPAG) in human plasma and urine. The sample preparation procedures involved simple protein precipitation for plasma and 10-fold dilution for urine. Each analytical run was completed within 15 min, with MPAG and MPA being eluted at 3.8 and 11.4 min, respectively. The optimized method showed good performance in terms of specificity, linearity, detection and quantitation limits, precision and accuracy. This assay was demonstrated to be applicable for clinical pharmacokinetic studies. © 2004 Elsevier B.V. All rights reserved.
Source Title: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
URI: https://scholarbank.nus.edu.sg/handle/10635/229453
ISSN: 1570-0232
1873-376X
DOI: 10.1016/j.jchromb.2004.02.020
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