Please use this identifier to cite or link to this item: https://doi.org/10.4252/wjsc.v10.i4.34
Title: NF-kappa B promotes the stem-like properties of leukemia cells by activation of LIN28B
Authors: Zhou, Jianbiao 
Chooi, Jing-Yuan 
Ching, Ying Qing 
Quah, Jessie Yiying 
Toh, Sabrina Hui-Min 
Ng, Yvonne 
Tan, Tuan Zea 
Chng, Wee-Joo 
Keywords: Science & Technology
Life Sciences & Biomedicine
Cell & Tissue Engineering
Cell Biology
Nuclear factor kappa B
LIN28B
Leukemia stem cell
Acute myeloid leukemia
ACUTE MYELOID-LEUKEMIA
PROGENITOR CELLS
CANCER
PROGRESSION
AML
Issue Date: 26-Apr-2018
Publisher: BAISHIDENG PUBLISHING GROUP INC
Citation: Zhou, Jianbiao, Chooi, Jing-Yuan, Ching, Ying Qing, Quah, Jessie Yiying, Toh, Sabrina Hui-Min, Ng, Yvonne, Tan, Tuan Zea, Chng, Wee-Joo (2018-04-26). NF-kappa B promotes the stem-like properties of leukemia cells by activation of LIN28B. WORLD JOURNAL OF STEM CELLS 10 (4) : 34-42. ScholarBank@NUS Repository. https://doi.org/10.4252/wjsc.v10.i4.34
Abstract: AIM To examine whether nuclear factor kappa B (NF-κB) activity regulates LIN28B expression and their roles in leukemia stem cell (LSC)-like properties. METHODS We used pharmacological inhibitor and cell viability assays to examine the relation between NF-κB and LIN28B. Western blot and qRT-PCR was employed to determine their protein and mRNA levels. Luciferase reporter was constructed and applied to explore the transcriptional regulation of LIN28B. We manipulated LIN28B level in acute myeloid leukemia (AML) cells and investigated LSC-like properties with colony forming and serial replating assays. RESULTS This study revealed the relationship between NF-κB and LIN28B in AML cells through drug inhibition and overexpression experiments. Notably, inhibition of NF-κB by pharmacological inhibitors reduced LIN28B expression and decreased cell proliferation. We demonstrated that NF-κB binds to the -819 to -811 region of LIN28B promoter, and transcriptionally regulates LIN28B expression. LIN28B protein was significantly elevated in NFκB1 transfected cells compared to vector control. Importantly, ectopic expression of LIN28B partially rescued the self-renewal capacity impaired by pharmacological inhibition of NF- κB activity. CONCLUSION These results uncover a regulatory signaling, NF-κB/ LIN28B, which plays a pivotal role in leukemia stem cell-like properties and it could serve as a promising intervening target for effective treatment of AML disease.
Source Title: WORLD JOURNAL OF STEM CELLS
URI: https://scholarbank.nus.edu.sg/handle/10635/229013
ISSN: 19480210
DOI: 10.4252/wjsc.v10.i4.34
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