Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41592-018-0139-3
Title: Reducing effects of particle adsorption to the air-water interface in cryo-EM
Authors: Noble, Alex J
Wei, Hui
Dandey, Venkata P
Zhang, Zhening
Tan, Yong Zi 
Potter, Clinton S
Carragher, Bridget
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemical Research Methods
Biochemistry & Molecular Biology
TILT-SERIES
CRYOELECTRON MICROSCOPY
RECONSTRUCTION
ALIGNMENT
CRYSTALS
SPOTITON
Issue Date: 1-Oct-2018
Publisher: NATURE PUBLISHING GROUP
Citation: Noble, Alex J, Wei, Hui, Dandey, Venkata P, Zhang, Zhening, Tan, Yong Zi, Potter, Clinton S, Carragher, Bridget (2018-10-01). Reducing effects of particle adsorption to the air-water interface in cryo-EM. NATURE METHODS 15 (10) : 793-+. ScholarBank@NUS Repository. https://doi.org/10.1038/s41592-018-0139-3
Abstract: Most protein particles prepared in vitreous ice for single-particle cryo-electron microscopy (cryo-EM) are adsorbed to air–water or substrate–water interfaces, which can cause the particles to adopt preferred orientations. By using a rapid plunge-freezing robot and nanowire grids, we were able to reduce some of the deleterious effects of the air–water interface by decreasing the dwell time of particles in thin liquid films. We demonstrated this by using single-particle cryo-EM and cryo-electron tomography (cryo-ET) to examine hemagglutinin, insulin receptor complex, and apoferritin.
Source Title: NATURE METHODS
URI: https://scholarbank.nus.edu.sg/handle/10635/227209
ISSN: 15487091
15487105
DOI: 10.1038/s41592-018-0139-3
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