Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41592-018-0139-3
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dc.titleReducing effects of particle adsorption to the air-water interface in cryo-EM
dc.contributor.authorNoble, Alex J
dc.contributor.authorWei, Hui
dc.contributor.authorDandey, Venkata P
dc.contributor.authorZhang, Zhening
dc.contributor.authorTan, Yong Zi
dc.contributor.authorPotter, Clinton S
dc.contributor.authorCarragher, Bridget
dc.date.accessioned2022-06-20T07:57:10Z
dc.date.available2022-06-20T07:57:10Z
dc.date.issued2018-10-01
dc.identifier.citationNoble, Alex J, Wei, Hui, Dandey, Venkata P, Zhang, Zhening, Tan, Yong Zi, Potter, Clinton S, Carragher, Bridget (2018-10-01). Reducing effects of particle adsorption to the air-water interface in cryo-EM. NATURE METHODS 15 (10) : 793-+. ScholarBank@NUS Repository. https://doi.org/10.1038/s41592-018-0139-3
dc.identifier.issn15487091
dc.identifier.issn15487105
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/227209
dc.description.abstractMost protein particles prepared in vitreous ice for single-particle cryo-electron microscopy (cryo-EM) are adsorbed to air–water or substrate–water interfaces, which can cause the particles to adopt preferred orientations. By using a rapid plunge-freezing robot and nanowire grids, we were able to reduce some of the deleterious effects of the air–water interface by decreasing the dwell time of particles in thin liquid films. We demonstrated this by using single-particle cryo-EM and cryo-electron tomography (cryo-ET) to examine hemagglutinin, insulin receptor complex, and apoferritin.
dc.language.isoen
dc.publisherNATURE PUBLISHING GROUP
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectBiochemical Research Methods
dc.subjectBiochemistry & Molecular Biology
dc.subjectTILT-SERIES
dc.subjectCRYOELECTRON MICROSCOPY
dc.subjectRECONSTRUCTION
dc.subjectALIGNMENT
dc.subjectCRYSTALS
dc.subjectSPOTITON
dc.typeArticle
dc.date.updated2022-06-18T14:56:28Z
dc.contributor.departmentDEPT OF BIOLOGICAL SCIENCES
dc.description.doi10.1038/s41592-018-0139-3
dc.description.sourcetitleNATURE METHODS
dc.description.volume15
dc.description.issue10
dc.description.page793-+
dc.published.statePublished
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