Please use this identifier to cite or link to this item:
https://doi.org/10.1038/s42003-022-03250-x
Title: | Non-coding RNA LEVER sequestration of PRC2 can mediate long range gene regulation | Authors: | Teo, Wei Wen Cao, Xinang Wu, Chan-Shuo Tan, Hong Kee Zhou, Qiling Gao, Chong Vanuytsel, Kim Kumar, Sara S Murphy, George J Koh Cheong Yang Henry Chai, Li Tenen, Daniel G |
Keywords: | Science & Technology Life Sciences & Biomedicine Biology Multidisciplinary Sciences Life Sciences & Biomedicine - Other Topics Science & Technology - Other Topics REPRESSIVE COMPLEX 2 READ ALIGNMENT CHROMATIN RECRUITMENT BINDING DNA |
Issue Date: | 11-Apr-2022 | Publisher: | NATURE PORTFOLIO | Citation: | Teo, Wei Wen, Cao, Xinang, Wu, Chan-Shuo, Tan, Hong Kee, Zhou, Qiling, Gao, Chong, Vanuytsel, Kim, Kumar, Sara S, Murphy, George J, Koh Cheong Yang Henry, Chai, Li, Tenen, Daniel G (2022-04-11). Non-coding RNA LEVER sequestration of PRC2 can mediate long range gene regulation. COMMUNICATIONS BIOLOGY 5 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/s42003-022-03250-x | Abstract: | Polycomb Repressive Complex 2 (PRC2) is an epigenetic regulator required for gene silencing during development. Although PRC2 is a well-established RNA-binding complex, the biological function of PRC2-RNA interaction has been controversial. Here, we study the gene-regulatory role of the inhibitory PRC2-RNA interactions. We report a nuclear long non-coding RNA, LEVER, which mapped 236 kb upstream of the β-globin cluster as confirmed by Nanopore sequencing. LEVER RNA interacts with PRC2 in its nascent form, and this prevents the accumulation of the H3K27 repressive histone marks within LEVER locus. Interestingly, the accessible LEVER chromatin, in turn, suppresses the chromatin interactions between the ε-globin locus and β-globin locus control region (LCR), resulting in a repressive effect on ε-globin gene expression. Our findings validate that the nascent RNA-PRC2 interaction inhibits local PRC2 function in situ. More importantly, we demonstrate that such a local process can in turn regulate the expression of neighboring genes. | Source Title: | COMMUNICATIONS BIOLOGY | URI: | https://scholarbank.nus.edu.sg/handle/10635/226696 | ISSN: | 23993642 23993642 |
DOI: | 10.1038/s42003-022-03250-x |
Appears in Collections: | Elements Staff Publications |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
Non-coding RNA LEVER sequestration of PRC2 can mediate long range gene regulation.pdf | 1.5 MB | Adobe PDF | OPEN | Published | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.