Please use this identifier to cite or link to this item: https://doi.org/10.1038/s42003-022-03250-x
Title: Non-coding RNA LEVER sequestration of PRC2 can mediate long range gene regulation
Authors: Teo, Wei Wen 
Cao, Xinang
Wu, Chan-Shuo 
Tan, Hong Kee 
Zhou, Qiling 
Gao, Chong
Vanuytsel, Kim
Kumar, Sara S
Murphy, George J
Koh Cheong Yang Henry 
Chai, Li
Tenen, Daniel G 
Keywords: Science & Technology
Life Sciences & Biomedicine
Biology
Multidisciplinary Sciences
Life Sciences & Biomedicine - Other Topics
Science & Technology - Other Topics
REPRESSIVE COMPLEX 2
READ ALIGNMENT
CHROMATIN
RECRUITMENT
BINDING
DNA
Issue Date: 11-Apr-2022
Publisher: NATURE PORTFOLIO
Citation: Teo, Wei Wen, Cao, Xinang, Wu, Chan-Shuo, Tan, Hong Kee, Zhou, Qiling, Gao, Chong, Vanuytsel, Kim, Kumar, Sara S, Murphy, George J, Koh Cheong Yang Henry, Chai, Li, Tenen, Daniel G (2022-04-11). Non-coding RNA LEVER sequestration of PRC2 can mediate long range gene regulation. COMMUNICATIONS BIOLOGY 5 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/s42003-022-03250-x
Abstract: Polycomb Repressive Complex 2 (PRC2) is an epigenetic regulator required for gene silencing during development. Although PRC2 is a well-established RNA-binding complex, the biological function of PRC2-RNA interaction has been controversial. Here, we study the gene-regulatory role of the inhibitory PRC2-RNA interactions. We report a nuclear long non-coding RNA, LEVER, which mapped 236 kb upstream of the β-globin cluster as confirmed by Nanopore sequencing. LEVER RNA interacts with PRC2 in its nascent form, and this prevents the accumulation of the H3K27 repressive histone marks within LEVER locus. Interestingly, the accessible LEVER chromatin, in turn, suppresses the chromatin interactions between the ε-globin locus and β-globin locus control region (LCR), resulting in a repressive effect on ε-globin gene expression. Our findings validate that the nascent RNA-PRC2 interaction inhibits local PRC2 function in situ. More importantly, we demonstrate that such a local process can in turn regulate the expression of neighboring genes.
Source Title: COMMUNICATIONS BIOLOGY
URI: https://scholarbank.nus.edu.sg/handle/10635/226696
ISSN: 23993642
23993642
DOI: 10.1038/s42003-022-03250-x
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