Please use this identifier to cite or link to this item:
https://doi.org/10.1073/pnas.1907904116
Title: | Addressing cellular heterogeneity in tumor and circulation for refined prognostication | Authors: | Lim, Su Bin Yeo, Trifanny Lee, Wen Di Bhagat, Ali Asgar S Tan, Swee Jin Tan, Daniel Shao Weng Lim, Wan-Teck Lim, Chwee Teck |
Keywords: | Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics microfluidics circulating biomarkers tumor heterogeneity GENE-EXPRESSION INTRATUMOR HETEROGENEITY HIGH-THROUGHPUT RNA-SEQ CELLS CANCER EVOLUTION SURVIVAL IMPACT RISK |
Issue Date: | 3-Sep-2019 | Publisher: | NATL ACAD SCIENCES | Citation: | Lim, Su Bin, Yeo, Trifanny, Lee, Wen Di, Bhagat, Ali Asgar S, Tan, Swee Jin, Tan, Daniel Shao Weng, Lim, Wan-Teck, Lim, Chwee Teck (2019-09-03). Addressing cellular heterogeneity in tumor and circulation for refined prognostication. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116 (36) : 17957-17962. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.1907904116 | Abstract: | Despite pronounced genomic and transcriptomic heterogeneity in non-small-cell lung cancer (NSCLC) not only between tumors, but also within a tumor, validation of clinically relevant gene signatures for prognostication has relied upon single-tissue samples, including 2 commercially available multigene tests (MGTs). Here we report an unanticipated impact of intratumor heterogeneity (ITH) on risk prediction of recurrence in NSCLC, underscoring the need for a better genomic strategy to refine prognostication. By leveraging label-free, inertial-focusing microfluidic approaches in retrieving circulating tumor cells (CTCs) at single-cell resolution, we further identified specific gene signatures with distinct expression profiles in CTCs from patients with differing metastatic potential. Notably, a refined prognostic risk model that reconciles the level of ITH and CTC-derived gene expression data outperformed the initial classifier in predicting recurrence-free survival (RFS). We propose tailored approaches to providing reliable risk estimates while accounting for ITH-driven variance in NSCLC. | Source Title: | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | URI: | https://scholarbank.nus.edu.sg/handle/10635/218558 | ISSN: | 00278424 10916490 |
DOI: | 10.1073/pnas.1907904116 |
Appears in Collections: | Elements Staff Publications |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
Addressing cellular heterogeneity in tumor and circulation for refined prognostication.pdf | 1.03 MB | Adobe PDF | OPEN | Published | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.