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Title: Salivary proteomic profiling identifies role of neutrophil extracellular traps formation in pregnancy gingivitis
Authors: Preethi Balan
Yap Seng Chong 
Qingsong Lin 
Teck Kwang Lim 
Tanujaa Suriyanarayanan 
Nadeeka Shiyamalee Udawatte
Mun Loke Wong 
Violeta Lopez 
Hong-Gu He 
Chaminda Jayampath Seneviratne 
Keywords: Pregnancy
Issue Date: 26-Apr-2021
Publisher: Taylor & Francis
Citation: Preethi Balan, Yap Seng Chong, Qingsong Lin, Teck Kwang Lim, Tanujaa Suriyanarayanan, Nadeeka Shiyamalee Udawatte, Mun Loke Wong, Violeta Lopez, Hong-Gu He, Chaminda Jayampath Seneviratne (2021-04-26). Salivary proteomic profiling identifies role of neutrophil extracellular traps formation in pregnancy gingivitis. Immunological Investigations 51 (01) : 103-119. ScholarBank@NUS Repository.
Abstract: Pregnancy gingivitis peaks during mid-pregnancy and resolves transiently towards the postpartum period. However, the role of maternal immune response in orchestrating gingival inflammation has not yet been fully understood. Hence, in this study, we examined the salivary protein profile during the three trimesters of pregnancy, in context to pregnancy gingivitis, employing iTRAQ-based quantitative proteomics. Unstimulated saliva was collected from 10 subjects in each trimester of pregnancy and postpartum period. Samples were analysed using iTRAQ analysis and ELISA and SEM was performed to validate results. Neutrophil mediated immune response was overrepresented in all three trimesters of pregnancy, despite the decrease in phagocytic responses during the second and third trimesters. ELISA showed a significantly higher Neutrophil Extracellular Traps (NETs) formation in the third trimester of pregnancy coinciding with the resolution of pregnancy gingivitis. The NETs-associated proteins (neutrophil elastase and myeloperoxidase) showed a positive correlation with estrogen hormones, which was also highest during the third trimester. Sex hormone-driven NETs formation could be the mainstay of defence that contributes to the remission of pregnancy gingivitis. This study has provided a new insight into the role of immune-modulation in pregnancy gingivitis, which will aid development of new therapeutics for managing pregnancy gingivitis in future.
Source Title: Immunological Investigations
ISSN: 0882-0139
DOI: 10.1080/08820139.2020.1810704
Appears in Collections:Staff Publications

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