Please use this identifier to cite or link to this item: https://doi.org/10.1126/sciadv.abe5940
Title: Catalytic amplification by transition-state molecular switches for direct and sensitive detection of SARS-CoV-2
Authors: Sundah, Noah R 
Natalia, Auginia 
Liu, Yu 
Ho, Nicholas RY 
Zhao, Haitao 
Chen, Yuan 
Miow, Qing Hao 
Wang, Yu 
Beh, Darius LL
Chew, Ka Lip
Chan, Douglas
Tambyah, Paul A 
Ong, Catherine WM 
Shao, Huilin
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
Issue Date: 1-Mar-2021
Publisher: AMER ASSOC ADVANCEMENT SCIENCE
Citation: Sundah, Noah R, Natalia, Auginia, Liu, Yu, Ho, Nicholas RY, Zhao, Haitao, Chen, Yuan, Miow, Qing Hao, Wang, Yu, Beh, Darius LL, Chew, Ka Lip, Chan, Douglas, Tambyah, Paul A, Ong, Catherine WM, Shao, Huilin (2021-03-01). Catalytic amplification by transition-state molecular switches for direct and sensitive detection of SARS-CoV-2. SCIENCE ADVANCES 7 (12). ScholarBank@NUS Repository. https://doi.org/10.1126/sciadv.abe5940
Abstract: Despite the importance of nucleic acid testing in managing the COVID-19 pandemic, current detection approaches remain limited due to their high complexity and extensive processing. Here, we describe a molecular nanotechnology that enables direct and sensitive detection of viral RNA targets in native clinical samples. The technology, termed catalytic amplification by transition-state molecular switch (CATCH), leverages DNA-enzyme hybrid complexes to form a molecular switch. By ratiometric tuning of its constituents, the multicomponent molecular switch is prepared in a hyperresponsive state—the transition state—that can be readily activated upon the binding of sparse RNA targets to turn on substantial enzymatic activity. CATCH thus achieves superior performance (~8 RNA copies/μl), direct fluorescence detection that bypasses all steps of PCR (<1 hour at room temperature), and versatile implementation (high-throughput 96-well format and portable microfluidic assay). When applied for clinical COVID-19 diagnostics, CATCH demonstrated direct and accurate detection in minimally processed patient swab samples.
Source Title: SCIENCE ADVANCES
URI: https://scholarbank.nus.edu.sg/handle/10635/216658
ISSN: 2375-2548
DOI: 10.1126/sciadv.abe5940
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