Please use this identifier to cite or link to this item:
https://doi.org/10.1126/sciadv.abe5940
DC Field | Value | |
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dc.title | Catalytic amplification by transition-state molecular switches for direct and sensitive detection of SARS-CoV-2 | |
dc.contributor.author | Sundah, Noah R | |
dc.contributor.author | Natalia, Auginia | |
dc.contributor.author | Liu, Yu | |
dc.contributor.author | Ho, Nicholas RY | |
dc.contributor.author | Zhao, Haitao | |
dc.contributor.author | Chen, Yuan | |
dc.contributor.author | Miow, Qing Hao | |
dc.contributor.author | Wang, Yu | |
dc.contributor.author | Beh, Darius LL | |
dc.contributor.author | Chew, Ka Lip | |
dc.contributor.author | Chan, Douglas | |
dc.contributor.author | Tambyah, Paul A | |
dc.contributor.author | Ong, Catherine WM | |
dc.contributor.author | Shao, Huilin | |
dc.date.accessioned | 2022-03-03T03:44:27Z | |
dc.date.available | 2022-03-03T03:44:27Z | |
dc.date.issued | 2021-03-01 | |
dc.identifier.citation | Sundah, Noah R, Natalia, Auginia, Liu, Yu, Ho, Nicholas RY, Zhao, Haitao, Chen, Yuan, Miow, Qing Hao, Wang, Yu, Beh, Darius LL, Chew, Ka Lip, Chan, Douglas, Tambyah, Paul A, Ong, Catherine WM, Shao, Huilin (2021-03-01). Catalytic amplification by transition-state molecular switches for direct and sensitive detection of SARS-CoV-2. SCIENCE ADVANCES 7 (12). ScholarBank@NUS Repository. https://doi.org/10.1126/sciadv.abe5940 | |
dc.identifier.issn | 2375-2548 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/216658 | |
dc.description.abstract | Despite the importance of nucleic acid testing in managing the COVID-19 pandemic, current detection approaches remain limited due to their high complexity and extensive processing. Here, we describe a molecular nanotechnology that enables direct and sensitive detection of viral RNA targets in native clinical samples. The technology, termed catalytic amplification by transition-state molecular switch (CATCH), leverages DNA-enzyme hybrid complexes to form a molecular switch. By ratiometric tuning of its constituents, the multicomponent molecular switch is prepared in a hyperresponsive state—the transition state—that can be readily activated upon the binding of sparse RNA targets to turn on substantial enzymatic activity. CATCH thus achieves superior performance (~8 RNA copies/μl), direct fluorescence detection that bypasses all steps of PCR (<1 hour at room temperature), and versatile implementation (high-throughput 96-well format and portable microfluidic assay). When applied for clinical COVID-19 diagnostics, CATCH demonstrated direct and accurate detection in minimally processed patient swab samples. | |
dc.language.iso | en | |
dc.publisher | AMER ASSOC ADVANCEMENT SCIENCE | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Multidisciplinary Sciences | |
dc.subject | Science & Technology - Other Topics | |
dc.type | Article | |
dc.date.updated | 2022-03-03T03:34:02Z | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.contributor.department | CIVIL ENGINEERING | |
dc.contributor.department | MEDICINE | |
dc.contributor.department | PHYSICS | |
dc.contributor.department | INST FOR HEALTH INNOVATION & TECHNOLOGY | |
dc.contributor.department | INSTITUTE OF SYSTEMS SCIENCE | |
dc.description.doi | 10.1126/sciadv.abe5940 | |
dc.description.sourcetitle | SCIENCE ADVANCES | |
dc.description.volume | 7 | |
dc.description.issue | 12 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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File | Description | Size | Format | Access Settings | Version | |
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Catalytic amplification by transition-state molecular switches for direct and sensitive detection of SARS-CoV-2.pdf | 2.35 MB | Adobe PDF | OPEN | Published | View/Download |
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