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Title: Aggregation-induced emission (AIE) nanoparticles labeled human embryonic stem cells (hESCs)-derived neurons for transplantation
Authors: Jang, Se Eun
Qiu, Lifeng 
Cai, Xiaolei 
Lee, Jolene Wei Ling
Zhang, Wei
Tan, Eng-King 
Liu, Bin 
Zeng, Li 
Keywords: Science & Technology
Engineering, Biomedical
Materials Science, Biomaterials
Materials Science
hESC-derived neurons
Microglia activation
in vivo tracking
Issue Date: 1-Apr-2021
Citation: Jang, Se Eun, Qiu, Lifeng, Cai, Xiaolei, Lee, Jolene Wei Ling, Zhang, Wei, Tan, Eng-King, Liu, Bin, Zeng, Li (2021-04-01). Aggregation-induced emission (AIE) nanoparticles labeled human embryonic stem cells (hESCs)-derived neurons for transplantation. BIOMATERIALS 271. ScholarBank@NUS Repository.
Abstract: Transplantation of differentiated neurons derived from either human embryonic stem cells (hESCs) or induced pluripotent stem cells (iPSCs) is an emerging therapeutic strategy for various neurodegenerative diseases. One important aspect of transplantation is the accessibility to track and control the activity of the stem cells-derived neurons post-transplantation. Recently, the characteristics of organic nanoparticles (NPs) with aggregation-induced emission (AIE) have emerged as efficient cell labeling reagents, where positive outcomes were observed in long-term cancer cell tracing in vivo. In the current study, we designed, synthesized, and analyzed the biocompatibility of AIE-NPs in cultured neurons such as in mouse neuronal progenitor cells (NPCs) and hESC-derived neurons. Our data demonstrated that AIE-NPs show high degree of penetration into cells and presented intracellular long-term retention in vitro without altering the neuronal proliferation, differentiation, and viability. Furthermore, we have tracked AIE-NPs labeled neuronal grafts in mouse brain striatum in various time points post-transplantation. We demonstrated prolonged cellular retention of AIE-NPs labeled neuronal grafts 1 month post-transplantation in mouse brain striatum. Lastly, we have shown activation of brain microglia in response to AIE-NPs labeled grafts. Together, these findings highlight the potential application of AIE-NPs in neuronal transplantation.
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2021.120747
Appears in Collections:Staff Publications

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Post-print Available on 01-04-2023

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