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Title: Functional regeneration of tendons using scaffolds with physical anisotropy engineered via microarchitectural manipulation
Authors: Wang, Z. 
Lee, W.J.
Koh, B.T.H.
Hong, M. 
Wang, W.
Lim, P.N. 
Feng, J.
Park, L.S.
Kim, M.
Thian, E.S. 
Issue Date: 2018
Publisher: American Association for the Advancement of Science
Citation: Wang, Z., Lee, W.J., Koh, B.T.H., Hong, M., Wang, W., Lim, P.N., Feng, J., Park, L.S., Kim, M., Thian, E.S. (2018). Functional regeneration of tendons using scaffolds with physical anisotropy engineered via microarchitectural manipulation. Science Advances 4 (10) : eaat4537. ScholarBank@NUS Repository.
Rights: Attribution-NonCommercial 4.0 International
Abstract: Structural and hierarchical anisotropy underlies the structure-function relationship of most living tissues. Attempts to exploit the interplay between cells and their immediate environment have rarely featured macroscale, threedimensional constructs required for clinical applications. Furthermore, compromises to biomechanical robustness during fabrication often limit the scaffold's relevance in translational medicine. We report a polymeric threedimensional scaffold with tendon-like mechanical properties and controlled anisotropic microstructures. The scaffold was composed of two distinct portions, which enabled high porosity while retaining tendon-like mechanical properties. When tenocytes were cultured in vitro on the scaffold, phenotypic markers of tenogenesis such as type-I collagen, decorin, and tenascin were significantly expressed over nonanisotropic controls. Moreover, highly aligned intracellular cytoskeletal network and high nuclear alignment efficiencies were observed, suggesting that microstructural anisotropy might play the epigenetic role of mechanotransduction. When implanted in an in vivo micropig model, a neotissue that formed over the scaffold resembled native tendon tissue in composition and structure. © 2018 The Authors.
Source Title: Science Advances
ISSN: 2375-2548
DOI: 10.1126/sciadv.aat4537
Rights: Attribution-NonCommercial 4.0 International
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