Please use this identifier to cite or link to this item:
Title: VCP/p97 is a proviral host factor for replication of chikungunya virus and other alphaviruses
Authors: Carissimo, G.
Chan, Y.-H.
Utt, A.
Chua, T.-K.
Bakar, F.A.
Merits, A.
Ng, L.F.P. 
Keywords: Alphavirus
Host factor
Proviral factor
Issue Date: 2019
Publisher: Frontiers Media S.A.
Citation: Carissimo, G., Chan, Y.-H., Utt, A., Chua, T.-K., Bakar, F.A., Merits, A., Ng, L.F.P. (2019). VCP/p97 is a proviral host factor for replication of chikungunya virus and other alphaviruses. Frontiers in Microbiology 10 (SEP) : 2236. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: The evolutionarily conserved AAA+ ATPase valosin-containing protein (VCP) was previously shown to be a proviral host factor for several viruses from different viral families such as Flaviviridae, Picornaviridae, and Herpesviridae. VCP was shown to affect trafficking of Sindbis virus receptor and functions as a component of Semliki Forest virus (SFV) replicase compartment. However, the role of this cellular protein was not evaluated during replication of alphaviruses including chikungunya virus (CHIKV). Using siRNA, chemical inhibitors, and trans-replication assays, we show here that VCP is a proviral factor involved in the replication of CHIKV. Immunofluorescence assays confirmed that VCP co-localized with non-structural replicase proteins but not with dsRNA foci possibly due to VCP epitope unavailability. VCP pro-viral role is also observed with other alphaviruses such as o抧yong抧yong virus (ONNV) and SFV in different human cell lines. VCP proviral roles on several viral families now extend to replication of alphaviruses CHIKV and ONNV, emphasizing the pivotal role of VCP in virus杊ost interaction biology. � 2019 Carissimo, Chan, Utt, Chua, Abu Bakar, Merits and Ng.
Source Title: Frontiers in Microbiology
ISSN: 1664302X
DOI: 10.3389/fmicb.2019.02236
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_3389_fmicb_2019_02236.pdf7.04 MBAdobe PDF




checked on Jan 26, 2023

Page view(s)

checked on Jan 26, 2023

Google ScholarTM



This item is licensed under a Creative Commons License Creative Commons