Please use this identifier to cite or link to this item: https://doi.org/10.3389/fmicb.2019.02236
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dc.titleVCP/p97 is a proviral host factor for replication of chikungunya virus and other alphaviruses
dc.contributor.authorCarissimo, G.
dc.contributor.authorChan, Y.-H.
dc.contributor.authorUtt, A.
dc.contributor.authorChua, T.-K.
dc.contributor.authorBakar, F.A.
dc.contributor.authorMerits, A.
dc.contributor.authorNg, L.F.P.
dc.date.accessioned2022-01-04T06:24:51Z
dc.date.available2022-01-04T06:24:51Z
dc.date.issued2019
dc.identifier.citationCarissimo, G., Chan, Y.-H., Utt, A., Chua, T.-K., Bakar, F.A., Merits, A., Ng, L.F.P. (2019). VCP/p97 is a proviral host factor for replication of chikungunya virus and other alphaviruses. Frontiers in Microbiology 10 (SEP) : 2236. ScholarBank@NUS Repository. https://doi.org/10.3389/fmicb.2019.02236
dc.identifier.issn1664302X
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/212939
dc.description.abstractThe evolutionarily conserved AAA+ ATPase valosin-containing protein (VCP) was previously shown to be a proviral host factor for several viruses from different viral families such as Flaviviridae, Picornaviridae, and Herpesviridae. VCP was shown to affect trafficking of Sindbis virus receptor and functions as a component of Semliki Forest virus (SFV) replicase compartment. However, the role of this cellular protein was not evaluated during replication of alphaviruses including chikungunya virus (CHIKV). Using siRNA, chemical inhibitors, and trans-replication assays, we show here that VCP is a proviral factor involved in the replication of CHIKV. Immunofluorescence assays confirmed that VCP co-localized with non-structural replicase proteins but not with dsRNA foci possibly due to VCP epitope unavailability. VCP pro-viral role is also observed with other alphaviruses such as o抧yong抧yong virus (ONNV) and SFV in different human cell lines. VCP proviral roles on several viral families now extend to replication of alphaviruses CHIKV and ONNV, emphasizing the pivotal role of VCP in virus杊ost interaction biology. � 2019 Carissimo, Chan, Utt, Chua, Abu Bakar, Merits and Ng.
dc.publisherFrontiers Media S.A.
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceScopus OA2019
dc.subjectAlphavirus
dc.subjectChikungunya
dc.subjectHost factor
dc.subjectProviral factor
dc.subjectVCP
dc.subjectVCP/p97
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.3389/fmicb.2019.02236
dc.description.sourcetitleFrontiers in Microbiology
dc.description.volume10
dc.description.issueSEP
dc.description.page2236
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