Please use this identifier to cite or link to this item: https://doi.org/10.1126/sciadv.aav8801
Title: Electrospray sample injection for single-particle imaging with x-ray lasers
Authors: Bielecki, J.
Hantke, M.F.
Daurer, B.J. 
Reddy, H.K.N.
Hasse, D.
Larsson, D.S.D.
Gunn, L.H.
Svenda, M.
Munke, A.
Sellberg, J.A.
Flueckiger, L.
Pietrini, A.
Nettelblad, C.
Lundholm, I.
Carlsson, G.
Okamoto, K.
Timneanu, N.
Westphal, D.
Kulyk, O.
Higashiura, A.
Van Der Schot, G.
Duane Loh, N.-T. 
Wysong, T.E.
Bostedt, C.
Gorkhover, T.
Iwan, B.
Seibert, M.M.
Osipov, T.
Walter, P.
Hart, P.
Bucher, M.
Ulmer, A.
Ray, D.
Carini, G.
Ferguson, K.R.
Andersson, I.
Andreasson, J.
Hajdu, J.
Maia, F.R.N.C.
Issue Date: 2019
Publisher: American Association for the Advancement of Science
Citation: Bielecki, J., Hantke, M.F., Daurer, B.J., Reddy, H.K.N., Hasse, D., Larsson, D.S.D., Gunn, L.H., Svenda, M., Munke, A., Sellberg, J.A., Flueckiger, L., Pietrini, A., Nettelblad, C., Lundholm, I., Carlsson, G., Okamoto, K., Timneanu, N., Westphal, D., Kulyk, O., Higashiura, A., Van Der Schot, G., Duane Loh, N.-T., Wysong, T.E., Bostedt, C., Gorkhover, T., Iwan, B., Seibert, M.M., Osipov, T., Walter, P., Hart, P., Bucher, M., Ulmer, A., Ray, D., Carini, G., Ferguson, K.R., Andersson, I., Andreasson, J., Hajdu, J., Maia, F.R.N.C. (2019). Electrospray sample injection for single-particle imaging with x-ray lasers. Science Advances 5 (5) : eaav8801. ScholarBank@NUS Repository. https://doi.org/10.1126/sciadv.aav8801
Rights: Attribution-NonCommercial 4.0 International
Abstract: The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution. © 2019 The Authors.
Source Title: Science Advances
URI: https://scholarbank.nus.edu.sg/handle/10635/212521
ISSN: 2375-2548
DOI: 10.1126/sciadv.aav8801
Rights: Attribution-NonCommercial 4.0 International
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