Please use this identifier to cite or link to this item: https://doi.org/10.1126/sciadv.aav8801
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dc.titleElectrospray sample injection for single-particle imaging with x-ray lasers
dc.contributor.authorBielecki, J.
dc.contributor.authorHantke, M.F.
dc.contributor.authorDaurer, B.J.
dc.contributor.authorReddy, H.K.N.
dc.contributor.authorHasse, D.
dc.contributor.authorLarsson, D.S.D.
dc.contributor.authorGunn, L.H.
dc.contributor.authorSvenda, M.
dc.contributor.authorMunke, A.
dc.contributor.authorSellberg, J.A.
dc.contributor.authorFlueckiger, L.
dc.contributor.authorPietrini, A.
dc.contributor.authorNettelblad, C.
dc.contributor.authorLundholm, I.
dc.contributor.authorCarlsson, G.
dc.contributor.authorOkamoto, K.
dc.contributor.authorTimneanu, N.
dc.contributor.authorWestphal, D.
dc.contributor.authorKulyk, O.
dc.contributor.authorHigashiura, A.
dc.contributor.authorVan Der Schot, G.
dc.contributor.authorDuane Loh, N.-T.
dc.contributor.authorWysong, T.E.
dc.contributor.authorBostedt, C.
dc.contributor.authorGorkhover, T.
dc.contributor.authorIwan, B.
dc.contributor.authorSeibert, M.M.
dc.contributor.authorOsipov, T.
dc.contributor.authorWalter, P.
dc.contributor.authorHart, P.
dc.contributor.authorBucher, M.
dc.contributor.authorUlmer, A.
dc.contributor.authorRay, D.
dc.contributor.authorCarini, G.
dc.contributor.authorFerguson, K.R.
dc.contributor.authorAndersson, I.
dc.contributor.authorAndreasson, J.
dc.contributor.authorHajdu, J.
dc.contributor.authorMaia, F.R.N.C.
dc.date.accessioned2021-12-29T05:48:33Z
dc.date.available2021-12-29T05:48:33Z
dc.date.issued2019
dc.identifier.citationBielecki, J., Hantke, M.F., Daurer, B.J., Reddy, H.K.N., Hasse, D., Larsson, D.S.D., Gunn, L.H., Svenda, M., Munke, A., Sellberg, J.A., Flueckiger, L., Pietrini, A., Nettelblad, C., Lundholm, I., Carlsson, G., Okamoto, K., Timneanu, N., Westphal, D., Kulyk, O., Higashiura, A., Van Der Schot, G., Duane Loh, N.-T., Wysong, T.E., Bostedt, C., Gorkhover, T., Iwan, B., Seibert, M.M., Osipov, T., Walter, P., Hart, P., Bucher, M., Ulmer, A., Ray, D., Carini, G., Ferguson, K.R., Andersson, I., Andreasson, J., Hajdu, J., Maia, F.R.N.C. (2019). Electrospray sample injection for single-particle imaging with x-ray lasers. Science Advances 5 (5) : eaav8801. ScholarBank@NUS Repository. https://doi.org/10.1126/sciadv.aav8801
dc.identifier.issn2375-2548
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/212521
dc.description.abstractThe possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution. © 2019 The Authors.
dc.publisherAmerican Association for the Advancement of Science
dc.rightsAttribution-NonCommercial 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.sourceScopus OA2019
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentPHYSICS
dc.description.doi10.1126/sciadv.aav8801
dc.description.sourcetitleScience Advances
dc.description.volume5
dc.description.issue5
dc.description.pageeaav8801
dc.published.statePublished
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