Please use this identifier to cite or link to this item:
Title: A Prototype Antibody-based Biosensor for Measurement of Salivary MMP-8 in Periodontitis using Surface Acoustic Wave Technology
Authors: Taylor, J.J.
Jaedicke, K.M.
van de Merwe, R.C.
Bissett, S.M.
Landsdowne, N.
Whall, K.M.
Pickering, K.
Thornton, V.
Lawson, V.
Yatsuda, H.
Kogai, T.
Shah, D.
Athey, D.
Preshaw, P.M. 
Issue Date: 2019
Publisher: Nature Publishing Group
Citation: Taylor, J.J., Jaedicke, K.M., van de Merwe, R.C., Bissett, S.M., Landsdowne, N., Whall, K.M., Pickering, K., Thornton, V., Lawson, V., Yatsuda, H., Kogai, T., Shah, D., Athey, D., Preshaw, P.M. (2019). A Prototype Antibody-based Biosensor for Measurement of Salivary MMP-8 in Periodontitis using Surface Acoustic Wave Technology. Scientific Reports 9 (1) : 11034. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Periodontitis is an economically important disease which is highly prevalent worldwide. Current diagnostic approaches are time-consuming and require interpretation of multiple aspects of clinical and radiographic assessment. Chair-side monitoring of inflammatory mediators of periodontitis could provide immediate information about disease activity, which can inform patient management. We aimed to develop a novel prototype biosensor to measure salivary matrix metalloproteinase-8 (MMP-8) using specific antibodies and surface acoustic wave (SAW) technology. The analytical performance of the prototype biosensor was compared to standard enzyme-linked immunosorbent assay (ELISA) using unstimulated saliva samples obtained from patients with periodontitis before and after non-surgical treatment (N = 58), patients with gingivitis (N = 54) and periodontally healthy volunteers (N = 65). Receiver operator characteristic (ROC) analysis for distinguishing periodontitis from health revealed an almost identical performance between the sensor and ELISA assays (area under curve values (AUC): ELISA 0.93; SAW 0.89). Furthermore, both analytical approaches yielded readouts which distinguished between heath, gingivitis and periodontitis, correlated identically with clinical measures of periodontal disease and recorded similar post-treatment decreases in salivary MMP-8 in periodontitis. The assay time for our prototype device is 20 minutes. The prototype SAW biosensor is a novel and rapid method of monitoring periodontitis which delivers similar analytical performance to conventional laboratory assays. © 2019, The Author(s).
Source Title: Scientific Reports
ISSN: 20452322
DOI: 10.1038/s41598-019-47513-w
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1038_s41598-019-47513-w.pdf1.48 MBAdobe PDF



Google ScholarTM



This item is licensed under a Creative Commons License Creative Commons