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https://doi.org/10.1038/s41598-019-47513-w
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dc.title | A Prototype Antibody-based Biosensor for Measurement of Salivary MMP-8 in Periodontitis using Surface Acoustic Wave Technology | |
dc.contributor.author | Taylor, J.J. | |
dc.contributor.author | Jaedicke, K.M. | |
dc.contributor.author | van de Merwe, R.C. | |
dc.contributor.author | Bissett, S.M. | |
dc.contributor.author | Landsdowne, N. | |
dc.contributor.author | Whall, K.M. | |
dc.contributor.author | Pickering, K. | |
dc.contributor.author | Thornton, V. | |
dc.contributor.author | Lawson, V. | |
dc.contributor.author | Yatsuda, H. | |
dc.contributor.author | Kogai, T. | |
dc.contributor.author | Shah, D. | |
dc.contributor.author | Athey, D. | |
dc.contributor.author | Preshaw, P.M. | |
dc.date.accessioned | 2021-12-29T04:05:02Z | |
dc.date.available | 2021-12-29T04:05:02Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Taylor, J.J., Jaedicke, K.M., van de Merwe, R.C., Bissett, S.M., Landsdowne, N., Whall, K.M., Pickering, K., Thornton, V., Lawson, V., Yatsuda, H., Kogai, T., Shah, D., Athey, D., Preshaw, P.M. (2019). A Prototype Antibody-based Biosensor for Measurement of Salivary MMP-8 in Periodontitis using Surface Acoustic Wave Technology. Scientific Reports 9 (1) : 11034. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-019-47513-w | |
dc.identifier.issn | 20452322 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/212232 | |
dc.description.abstract | Periodontitis is an economically important disease which is highly prevalent worldwide. Current diagnostic approaches are time-consuming and require interpretation of multiple aspects of clinical and radiographic assessment. Chair-side monitoring of inflammatory mediators of periodontitis could provide immediate information about disease activity, which can inform patient management. We aimed to develop a novel prototype biosensor to measure salivary matrix metalloproteinase-8 (MMP-8) using specific antibodies and surface acoustic wave (SAW) technology. The analytical performance of the prototype biosensor was compared to standard enzyme-linked immunosorbent assay (ELISA) using unstimulated saliva samples obtained from patients with periodontitis before and after non-surgical treatment (N = 58), patients with gingivitis (N = 54) and periodontally healthy volunteers (N = 65). Receiver operator characteristic (ROC) analysis for distinguishing periodontitis from health revealed an almost identical performance between the sensor and ELISA assays (area under curve values (AUC): ELISA 0.93; SAW 0.89). Furthermore, both analytical approaches yielded readouts which distinguished between heath, gingivitis and periodontitis, correlated identically with clinical measures of periodontal disease and recorded similar post-treatment decreases in salivary MMP-8 in periodontitis. The assay time for our prototype device is 20 minutes. The prototype SAW biosensor is a novel and rapid method of monitoring periodontitis which delivers similar analytical performance to conventional laboratory assays. © 2019, The Author(s). | |
dc.publisher | Nature Publishing Group | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Scopus OA2019 | |
dc.type | Article | |
dc.contributor.department | DEAN'S OFFICE (DENTISTRY) | |
dc.description.doi | 10.1038/s41598-019-47513-w | |
dc.description.sourcetitle | Scientific Reports | |
dc.description.volume | 9 | |
dc.description.issue | 1 | |
dc.description.page | 11034 | |
Appears in Collections: | Staff Publications Elements |
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