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Title: Agrobacterium Delivers Anchorage Protein VirE3 for Companion VirE2 to Aggregate at Host Entry Sites for T-DNA Protection
Authors: Li, X. 
Tu, H.
Pan, S.Q.
Keywords: Agrobacterium
anchorage protein
plasma membrane
Issue Date: 2018
Publisher: Elsevier B.V.
Citation: Li, X., Tu, H., Pan, S.Q. (2018). Agrobacterium Delivers Anchorage Protein VirE3 for Companion VirE2 to Aggregate at Host Entry Sites for T-DNA Protection. Cell Reports 25 (2) : 302-311000000. ScholarBank@NUS Repository.
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
Abstract: Agrobacterium tumefaciens transfers oncogenic DNA (T-DNA) and effector proteins into various host plants. T-DNA is generated inside the bacteria and subsequently delivered into plant cells along with the companion effectors VirD2, VirE2, and VirE3. However, it is not clear how the T-complex consisting of VirD2 and VirE2 is assembled inside plant cells. Here, we report that the effector protein VirE3 localized to plant plasma membranes as an anchorage through a conserved ?-helical-bundle domain. VirE3 interacted with itself and enabled VirE2 accumulation at host entry sites through direct interactions. VirE3 was critical for VirE2 function in T-DNA protection. Our data indicate that VirE3 functions as a previously unrecognized anchorage protein consisting of membrane-binding, self-interacting, and VirE2-interacting domains. Both VirE2 and VirE3 are conserved among Agrobacterium and rhizobia species but not other organisms, suggesting that a group of anchorage proteins have been generated through evolution to facilitate the nucleoprotein assembly at plant membranes. Agrobacterium transfers T-DNA and virulence proteins into host cells. Li et al. show that a virulence protein, VirE3, anchors its companion protein VirE2 on host membranes at the entrance to facilitate T-DNA protection. VirE3 represents a class of proteins, anchorage proteins, which are present only in agrobacteria and rhizobia. © 2018 The Authors
Source Title: Cell Reports
ISSN: 22111247
DOI: 10.1016/j.celrep.2018.09.023
Rights: Attribution-NonCommercial-NoDerivatives 4.0 International
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