Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.celrep.2018.09.023
DC Field | Value | |
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dc.title | Agrobacterium Delivers Anchorage Protein VirE3 for Companion VirE2 to Aggregate at Host Entry Sites for T-DNA Protection | |
dc.contributor.author | Li, X. | |
dc.contributor.author | Tu, H. | |
dc.contributor.author | Pan, S.Q. | |
dc.date.accessioned | 2021-12-28T10:02:46Z | |
dc.date.available | 2021-12-28T10:02:46Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Li, X., Tu, H., Pan, S.Q. (2018). Agrobacterium Delivers Anchorage Protein VirE3 for Companion VirE2 to Aggregate at Host Entry Sites for T-DNA Protection. Cell Reports 25 (2) : 302-311000000. ScholarBank@NUS Repository. https://doi.org/10.1016/j.celrep.2018.09.023 | |
dc.identifier.issn | 22111247 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/212125 | |
dc.description.abstract | Agrobacterium tumefaciens transfers oncogenic DNA (T-DNA) and effector proteins into various host plants. T-DNA is generated inside the bacteria and subsequently delivered into plant cells along with the companion effectors VirD2, VirE2, and VirE3. However, it is not clear how the T-complex consisting of VirD2 and VirE2 is assembled inside plant cells. Here, we report that the effector protein VirE3 localized to plant plasma membranes as an anchorage through a conserved ?-helical-bundle domain. VirE3 interacted with itself and enabled VirE2 accumulation at host entry sites through direct interactions. VirE3 was critical for VirE2 function in T-DNA protection. Our data indicate that VirE3 functions as a previously unrecognized anchorage protein consisting of membrane-binding, self-interacting, and VirE2-interacting domains. Both VirE2 and VirE3 are conserved among Agrobacterium and rhizobia species but not other organisms, suggesting that a group of anchorage proteins have been generated through evolution to facilitate the nucleoprotein assembly at plant membranes. Agrobacterium transfers T-DNA and virulence proteins into host cells. Li et al. show that a virulence protein, VirE3, anchors its companion protein VirE2 on host membranes at the entrance to facilitate T-DNA protection. VirE3 represents a class of proteins, anchorage proteins, which are present only in agrobacteria and rhizobia. © 2018 The Authors | |
dc.publisher | Elsevier B.V. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Scopus OA2018 | |
dc.subject | Agrobacterium | |
dc.subject | anchorage protein | |
dc.subject | plasma membrane | |
dc.subject | T-complex | |
dc.subject | T-DNA | |
dc.subject | VirE2 | |
dc.subject | VirE3 | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1016/j.celrep.2018.09.023 | |
dc.description.sourcetitle | Cell Reports | |
dc.description.volume | 25 | |
dc.description.issue | 2 | |
dc.description.page | 302-311000000 | |
Appears in Collections: | Staff Publications Elements |
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