Please use this identifier to cite or link to this item:
Title: Differential epithelial and stromal protein profiles in cone and non-cone regions of keratoconus corneas
Authors: Yam, G.H.-F. 
Fuest, M.
Zhou, L.
Liu, Y.-C. 
Deng, L.
Chan, A.S.-Y. 
Ong, H.S.
Khor, W.-B.
Ang, M. 
Mehta, J.S. 
Issue Date: 2019
Publisher: Nature Publishing Group
Citation: Yam, G.H.-F., Fuest, M., Zhou, L., Liu, Y.-C., Deng, L., Chan, A.S.-Y., Ong, H.S., Khor, W.-B., Ang, M., Mehta, J.S. (2019). Differential epithelial and stromal protein profiles in cone and non-cone regions of keratoconus corneas. Scientific Reports 9 (1) : 2965. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Keratoconus (KC) is an ectatic corneal disease characterized by progressive thinning and irregular astigmatism, and a leading indication for corneal transplantation. KC-associated changes have been demonstrated for the entire cornea, but the pathological thinning and mechanical weakening is usually localized. We performed quantitative proteomics using Sequential Windowed Acquisition of All Theoretical Fragment Ion Mass Spectrometry (SWATH-MS) to analyze epithelial and stromal changes between the topographically-abnormal cone and topographically-normal non-cone regions of advanced KC corneas, compared to age-matched normal corneas. Expression of 20 epithelial and 14 stromal proteins was significantly altered (?2 or ?0.5-fold) between cone and non-cone in all 4 KC samples. Ingenuity pathway analysis illustrated developmental and metabolic disorders for the altered epithelial proteome with mitochondrion as the significant gene ontology (GO) term. The differential stromal proteome was related to cellular assembly, tissue organization and connective tissue disorders with endoplasmic reticulum protein folding as the significant GO term. Validation of selected protein expression was performed on archived KC, non-KC and normal corneal specimens by immunohistochemistry. This is the first time to show that KC-associated proteome changes were not limited to the topographically-thinner and mechanically-weakened cone but also non-cone region with normal topography, indicating a peripheral involvement in KC development. © 2019, The Author(s).
Source Title: Scientific Reports
ISSN: 20452322
DOI: 10.1038/s41598-019-39182-6
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1038_s41598-019-39182-6.pdf2.65 MBAdobe PDF




checked on May 13, 2022

Page view(s)

checked on May 12, 2022

Google ScholarTM



This item is licensed under a Creative Commons License Creative Commons