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Title: Subtyping of circulating exosome-bound amyloid ? reflects brain plaque deposition
Authors: Lim, C.Z.J.
Zhang, Y.
Chen, Y.
Zhao, H. 
Stephenson, M.C.
Ho, N.R.Y.
Chen, Y. 
Chung, J.
Reilhac, A. 
Loh, T.P.
Chen, C.L.H. 
Shao, H. 
Issue Date: 2019
Publisher: Nature Publishing Group
Citation: Lim, C.Z.J., Zhang, Y., Chen, Y., Zhao, H., Stephenson, M.C., Ho, N.R.Y., Chen, Y., Chung, J., Reilhac, A., Loh, T.P., Chen, C.L.H., Shao, H. (2019). Subtyping of circulating exosome-bound amyloid ? reflects brain plaque deposition. Nature Communications 10 (1) : 1144. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Despite intense interests in developing blood measurements of Alzheimer’s disease (AD), the progress has been confounded by limited sensitivity and poor correlation to brain pathology. Here, we present a dedicated analytical platform for measuring different populations of circulating amyloid ? (A?) proteins – exosome-bound vs. unbound – directly from blood. The technology, termed amplified plasmonic exosome (APEX), leverages in situ enzymatic conversion of localized optical deposits and double-layered plasmonic nanostructures to enable sensitive, multiplexed population analysis. It demonstrates superior sensitivity (~200 exosomes), and enables diverse target co-localization in exosomes. Employing the platform, we find that prefibrillar A? aggregates preferentially bind with exosomes. We thus define a population of A? as exosome-bound (A?42+ CD63+) and measure its abundance directly from AD and control blood samples. As compared to the unbound or total circulating A?, the exosome-bound A? measurement could better reflect PET imaging of brain amyloid plaques and differentiate various clinical groups. © 2019, The Author(s).
Source Title: Nature Communications
ISSN: 20411723
DOI: 10.1038/s41467-019-09030-2
Rights: Attribution 4.0 International
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