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|Title:||Subtyping of circulating exosome-bound amyloid ? reflects brain plaque deposition||Authors:||Lim, C.Z.J.
|Issue Date:||2019||Publisher:||Nature Publishing Group||Citation:||Lim, C.Z.J., Zhang, Y., Chen, Y., Zhao, H., Stephenson, M.C., Ho, N.R.Y., Chen, Y., Chung, J., Reilhac, A., Loh, T.P., Chen, C.L.H., Shao, H. (2019). Subtyping of circulating exosome-bound amyloid ? reflects brain plaque deposition. Nature Communications 10 (1) : 1144. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-019-09030-2||Rights:||Attribution 4.0 International||Abstract:||Despite intense interests in developing blood measurements of Alzheimer’s disease (AD), the progress has been confounded by limited sensitivity and poor correlation to brain pathology. Here, we present a dedicated analytical platform for measuring different populations of circulating amyloid ? (A?) proteins – exosome-bound vs. unbound – directly from blood. The technology, termed amplified plasmonic exosome (APEX), leverages in situ enzymatic conversion of localized optical deposits and double-layered plasmonic nanostructures to enable sensitive, multiplexed population analysis. It demonstrates superior sensitivity (~200 exosomes), and enables diverse target co-localization in exosomes. Employing the platform, we find that prefibrillar A? aggregates preferentially bind with exosomes. We thus define a population of A? as exosome-bound (A?42+ CD63+) and measure its abundance directly from AD and control blood samples. As compared to the unbound or total circulating A?, the exosome-bound A? measurement could better reflect PET imaging of brain amyloid plaques and differentiate various clinical groups. © 2019, The Author(s).||Source Title:||Nature Communications||URI:||https://scholarbank.nus.edu.sg/handle/10635/212097||ISSN:||20411723||DOI:||10.1038/s41467-019-09030-2||Rights:||Attribution 4.0 International|
|Appears in Collections:||Staff Publications|
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