Please use this identifier to cite or link to this item: https://doi.org/10.7150/thno.65302
Title: Mucosal microbiome associates with progression to gastric cancer
Authors: PNG CHIN WEN 
LEE WEI JIE JONATHAN 
CHUA SHIJIA JOY 
Feng Zhu 
Gastric Consortium
Yeoh, K.-G. 
Zhang, Yongliang 
Keywords: Science & Technology
Life Sciences & Biomedicine
Medicine, Research & Experimental
Research & Experimental Medicine
gastric cancer
microbiome
intestinal metaplasia
early gastric neoplasia
Helicobacter pylori
UNIDENTIFIED CURVED BACILLI
HELICOBACTER-PYLORI
ERADICATION
PROTECTION
RESISTANCE
INFECTION
BACTERIA
REVEALS
STOMACH
RISK
Issue Date: 1-Jan-2022
Publisher: Ivyspring International Publisher.
Citation: PNG CHIN WEN, LEE WEI JIE JONATHAN, CHUA SHIJIA JOY, Feng Zhu, Gastric Consortium, Yeoh, K.-G., Zhang, Yongliang (2022-01-01). Mucosal microbiome associates with progression to gastric cancer. THERANOSTICS 12 (1) : 48-58. ScholarBank@NUS Repository. https://doi.org/10.7150/thno.65302
Abstract: Background & Aims: Dysbiosis is associated with gastric cancer (GC) development. However, no longitudinal study was carried out to identify key bacteria that could predict for GC progression. Here, we aimed to investigate changes in bacterial metagenome prior to GC and develop a microbiome-based predictive model to accurately classify patients at risk of GC. Methods: Bacterial 16S rDNA was sequenced from 89 gastric antral biopsies obtained from 43 participants. This study was nested in a prospective, longitudinal study, whereby study participants underwent screening gastroscopy, with further 1-2 yearly surveillance gastroscopies for at least 5 years. Putative bacterial taxonomic and functional features associated with GC carcinogenesis were identified by comparing between controls, patients with gastric intestinal metaplasia (IM) and patients with early gastric neoplasia (EGN). Results: Patients with EGN had enrichment of Proteobacteria (in particular Proteus genus) and depletion of Bacteroidetes (in particular S24-7 family) in their gastric mucosa. Sequencing identified more patients with Helicobacter pylori compared to histopathological assessment, while H. pylori was also significantly enriched in EGN. Furthermore, a total of 261 functional features, attributing to 97 KEGG pathways were differentially abundant at baseline between patients who subsequent developed EGN (n = 13/39) and those who did not. At the same time, a constellation of six microbial taxonomic features present at baseline, provided the highest classifying power for subsequent EGN (AUC = 0.82). Conclusion: Our study highlights early microbial changes associated with GC carcinogenesis, suggesting a potential role for prospective microbiome surveillance for GC.
Source Title: THERANOSTICS
URI: https://scholarbank.nus.edu.sg/handle/10635/211399
ISSN: 1838-7640
DOI: 10.7150/thno.65302
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