Please use this identifier to cite or link to this item:
https://doi.org/10.1038/s41467-019-10448-x
Title: | Coherent diffractive imaging of microtubules using an X-ray laser | Authors: | Brändén, G. Hammarin, G. Harimoorthy, R. Johansson, A. Arnlund, D. Malmerberg, E. Barty, A. Tångefjord, S. Berntsen, P. DePonte, D.P. Seuring, C. White, T.A. Stellato, F. Bean, R. Beyerlein, K.R. Chavas, L.M.G. Fleckenstein, H. Gati, C. Ghoshdastider, U. Gumprecht, L. Oberthür, D. Popp, D. Seibert, M. Tilp, T. Messerschmidt, M. Williams, G.J. Loh, N.D. Chapman, H.N. Zwart, P. Liang, M. Boutet, S. Robinson, R.C. Neutze, R. |
Issue Date: | 2019 | Publisher: | Nature Publishing Group | Citation: | Brändén, G., Hammarin, G., Harimoorthy, R., Johansson, A., Arnlund, D., Malmerberg, E., Barty, A., Tångefjord, S., Berntsen, P., DePonte, D.P., Seuring, C., White, T.A., Stellato, F., Bean, R., Beyerlein, K.R., Chavas, L.M.G., Fleckenstein, H., Gati, C., Ghoshdastider, U., Gumprecht, L., Oberthür, D., Popp, D., Seibert, M., Tilp, T., Messerschmidt, M., Williams, G.J., Loh, N.D., Chapman, H.N., Zwart, P., Liang, M., Boutet, S., Robinson, R.C., Neutze, R. (2019). Coherent diffractive imaging of microtubules using an X-ray laser. Nature Communications 10 (1) : 2589. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-019-10448-x | Rights: | Attribution 4.0 International | Abstract: | X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate approach: the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2 nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4 nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature. © 2019, The Author(s). | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/210697 | ISSN: | 20411723 | DOI: | 10.1038/s41467-019-10448-x | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
10_1038_s41467-019-10448-x.pdf | 3.82 MB | Adobe PDF | OPEN | None | View/Download |
This item is licensed under a Creative Commons License