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https://doi.org/10.1038/s41467-019-10448-x
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dc.title | Coherent diffractive imaging of microtubules using an X-ray laser | |
dc.contributor.author | Brändén, G. | |
dc.contributor.author | Hammarin, G. | |
dc.contributor.author | Harimoorthy, R. | |
dc.contributor.author | Johansson, A. | |
dc.contributor.author | Arnlund, D. | |
dc.contributor.author | Malmerberg, E. | |
dc.contributor.author | Barty, A. | |
dc.contributor.author | Tångefjord, S. | |
dc.contributor.author | Berntsen, P. | |
dc.contributor.author | DePonte, D.P. | |
dc.contributor.author | Seuring, C. | |
dc.contributor.author | White, T.A. | |
dc.contributor.author | Stellato, F. | |
dc.contributor.author | Bean, R. | |
dc.contributor.author | Beyerlein, K.R. | |
dc.contributor.author | Chavas, L.M.G. | |
dc.contributor.author | Fleckenstein, H. | |
dc.contributor.author | Gati, C. | |
dc.contributor.author | Ghoshdastider, U. | |
dc.contributor.author | Gumprecht, L. | |
dc.contributor.author | Oberthür, D. | |
dc.contributor.author | Popp, D. | |
dc.contributor.author | Seibert, M. | |
dc.contributor.author | Tilp, T. | |
dc.contributor.author | Messerschmidt, M. | |
dc.contributor.author | Williams, G.J. | |
dc.contributor.author | Loh, N.D. | |
dc.contributor.author | Chapman, H.N. | |
dc.contributor.author | Zwart, P. | |
dc.contributor.author | Liang, M. | |
dc.contributor.author | Boutet, S. | |
dc.contributor.author | Robinson, R.C. | |
dc.contributor.author | Neutze, R. | |
dc.date.accessioned | 2021-12-16T07:44:37Z | |
dc.date.available | 2021-12-16T07:44:37Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Brändén, G., Hammarin, G., Harimoorthy, R., Johansson, A., Arnlund, D., Malmerberg, E., Barty, A., Tångefjord, S., Berntsen, P., DePonte, D.P., Seuring, C., White, T.A., Stellato, F., Bean, R., Beyerlein, K.R., Chavas, L.M.G., Fleckenstein, H., Gati, C., Ghoshdastider, U., Gumprecht, L., Oberthür, D., Popp, D., Seibert, M., Tilp, T., Messerschmidt, M., Williams, G.J., Loh, N.D., Chapman, H.N., Zwart, P., Liang, M., Boutet, S., Robinson, R.C., Neutze, R. (2019). Coherent diffractive imaging of microtubules using an X-ray laser. Nature Communications 10 (1) : 2589. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-019-10448-x | |
dc.identifier.issn | 20411723 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/210697 | |
dc.description.abstract | X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has allowed high-resolution structures to be determined from micro-meter sized crystals, whereas single particle coherent X-ray imaging requires development to extend the resolution beyond a few tens of nanometers. Here we describe an intermediate approach: the XFEL imaging of biological assemblies with helical symmetry. We collected X-ray scattering images from samples of microtubules injected across an XFEL beam using a liquid microjet, sorted these images into class averages, merged these data into a diffraction pattern extending to 2 nm resolution, and reconstructed these data into a projection image of the microtubule. Details such as the 4 nm tubulin monomer became visible in this reconstruction. These results illustrate the potential of single-molecule X-ray imaging of biological assembles with helical symmetry at room temperature. © 2019, The Author(s). | |
dc.publisher | Nature Publishing Group | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Scopus OA2019 | |
dc.type | Article | |
dc.contributor.department | PHYSICS | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.doi | 10.1038/s41467-019-10448-x | |
dc.description.sourcetitle | Nature Communications | |
dc.description.volume | 10 | |
dc.description.issue | 1 | |
dc.description.page | 2589 | |
Appears in Collections: | Elements Staff Publications |
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