Please use this identifier to cite or link to this item: https://doi.org/10.18632/aging.203202
Title: Spatiotemporal dynamics of gamma H2AX in the mouse brain after acute irradiation at different postnatal days with special reference to the dentate gyrus of the hippocampus
Authors: Tang, Feng Ru 
Liu, Lian
Wang, Hong 
Ho, Kimberly Jen Ni 
Sethi, Gautam 
Keywords: Science & Technology
Life Sciences & Biomedicine
Cell Biology
Geriatrics & Gerontology
gamma H2AX
radiation
spatiotemporal dynamics
brain
dentate gyrus
DEFECTIVE-DNA REPAIR
DOUBLE-STRAND BREAK
IONIZING-RADIATION
HISTONE H2AX
LONG-TERM
ALZHEIMERS-DISEASE
ENDOTHELIAL-CELLS
HYPOXIA-ISCHEMIA
RAT HIPPOCAMPUS
DAMAGE
Issue Date: 30-Jun-2021
Publisher: IMPACT JOURNALS LLC
Citation: Tang, Feng Ru, Liu, Lian, Wang, Hong, Ho, Kimberly Jen Ni, Sethi, Gautam (2021-06-30). Spatiotemporal dynamics of gamma H2AX in the mouse brain after acute irradiation at different postnatal days with special reference to the dentate gyrus of the hippocampus. AGING-US 13 (12) : 15815-15832. ScholarBank@NUS Repository. https://doi.org/10.18632/aging.203202
Abstract: Gamma H2A histone family member X (γH2AX) is a molecular marker of aging and disease. However, radiosensitivity of the different brain cells, including neurons, glial cells, cells in cerebrovascular system, epithelial cells in pia mater, ependymal cells lining the ventricles of the brain in immature animals at different postnatal days remains unknown. Whether radiation-induced γH2AX foci in immature brain persist in adult animals still needs to be investigated. Hence, using a mouse model, we showed an extensive postnatal age-dependent induction of γH2AX foci in different brain regions at 1 day after whole body gamma irradiation with 5Gy at postnatal day 3 (P3), P10 and P21. P3 mouse brain epithelial cells in pia mater, glial cells in white matter and cells in cerebrovascular system were more radiosensitive at one day after radiation exposure than those from P10 and P21 mice. Persistent DNA damage foci (PDDF) were consistently demonstrated in the brain at 120 days and 15 months after irradiation at P3, P10 and P21, and these mice had shortened lifespan compared to the age-matched control. Our results suggest that early life irradiation-induced PDDF at later stages of animal life may be related to the brain aging and shortened life expectancy of irradiated animals.
Source Title: AGING-US
URI: https://scholarbank.nus.edu.sg/handle/10635/210297
ISSN: 1945-4589
DOI: 10.18632/aging.203202
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