Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-019-13414-9
Title: Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases
Authors: Su, N.
Byrd, A.K.
Bharath, S.R.
Yang, O.
Jia, Y.
Tang, X.
Ha, T.
Raney, K.D.
Song, H. 
Issue Date: 2019
Publisher: Nature Research
Citation: Su, N., Byrd, A.K., Bharath, S.R., Yang, O., Jia, Y., Tang, X., Ha, T., Raney, K.D., Song, H. (2019). Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases. Nature Communications 10 (1) : 5375. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-019-13414-9
Rights: Attribution 4.0 International
Abstract: Pif1 plays multiple roles in maintaining genome stability and preferentially unwinds forked dsDNA, but the mechanism by which Pif1 unwinds forked dsDNA remains elusive. Here we report the structure of Bacteroides sp Pif1 (BaPif1) in complex with a symmetrical double forked dsDNA. Two interacting BaPif1 molecules are bound to each fork of the partially unwound dsDNA, and interact with the 5? arm and 3? ss/dsDNA respectively. Each of the two BaPif1 molecules is an active helicase and their interaction may regulate their helicase activities. The binding of BaPif1 to the 5? arm causes a sharp bend in the 5? ss/dsDNA junction, consequently breaking the first base-pair. BaPif1 bound to the 3? ss/dsDNA junction impacts duplex unwinding by stabilizing the unpaired first base-pair and engaging the second base-pair poised for breaking. Our results provide an unprecedented insight into how two BaPif1 coordinate with each other to unwind the forked dsDNA. © 2019, The Author(s).
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/209897
ISSN: 2041-1723
DOI: 10.1038/s41467-019-13414-9
Rights: Attribution 4.0 International
Appears in Collections:Elements
Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1038_s41467-019-13414-9.pdf2.24 MBAdobe PDF

OPEN

NoneView/Download

SCOPUSTM   
Citations

17
checked on Dec 2, 2022

Page view(s)

76
checked on Dec 1, 2022

Google ScholarTM

Check

Altmetric


This item is licensed under a Creative Commons License Creative Commons