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Title: MNK1 and MNK2 enforce expression of E2F1, FOXM1, and WEE1 to drive soft tissue sarcoma
Authors: Ke, Xin-Yu
Chen, Ye
Tham, Valarie Yu-Yan
Lin, Ruby Yu-Tong
Dakle, Pushkar 
Nacro, Kassoum
Puhaindran, Mark Edward
Houghton, Peter
Pang, Angela
Lee, Victor Kwanmin 
Ding, Ling-Wen 
Gery, Sigal
Hill, Jeffrey
Chen, Leilei
Xu, Liang
Koeffler, H Phillip
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Cell Biology
Genetics & Heredity
Issue Date: 9-Feb-2021
Citation: Ke, Xin-Yu, Chen, Ye, Tham, Valarie Yu-Yan, Lin, Ruby Yu-Tong, Dakle, Pushkar, Nacro, Kassoum, Puhaindran, Mark Edward, Houghton, Peter, Pang, Angela, Lee, Victor Kwanmin, Ding, Ling-Wen, Gery, Sigal, Hill, Jeffrey, Chen, Leilei, Xu, Liang, Koeffler, H Phillip (2021-02-09). MNK1 and MNK2 enforce expression of E2F1, FOXM1, and WEE1 to drive soft tissue sarcoma. ONCOGENE 40 (10) : 1851-1867. ScholarBank@NUS Repository.
Abstract: Soft tissue sarcoma (STS) is a heterogeneous disease that arises from connective tissues. Clinical outcome of patients with advanced tumors especially de-differentiated liposarcoma and uterine leiomyosarcoma remains unsatisfactory, despite intensive treatment regimens including maximal surgical resection, radiation, and chemotherapy. MAP kinase-interacting serine/threonine-protein kinase 1 and 2 (MNK1/2) have been shown to contribute to oncogenic translation via phosphorylation of eukaryotic translation initiation factor 4E (eIF4E). However, little is known about the role of MNK1/2 and their downstream targets in STS. In this study, we show that depletion of either MNK1 or MNK2 suppresses cell viability, anchorage-independent growth, and tumorigenicity of STS cells. We also identify a compelling antiproliferative efficacy of a novel, selective MNK inhibitor ETC-168. Cellular responsiveness of STS cells to ETC-168 correlates positively with that of phosphorylated ribosomal protein S6 (RPS6). Mirroring MNK1/2 silencing, ETC-168 treatment strongly blocks eIF4E phosphorylation and represses expression of sarcoma-driving onco-proteins including E2F1, FOXM1, and WEE1. Moreover, combination of ETC-168 and MCL1 inhibitor S63845 exerts a synergistic antiproliferative activity against STS cells. In summary, our study reveals crucial roles of MNK1/2 and their downstream targets in STS tumorigenesis. Our data encourage further clinical translation of MNK inhibitors for STS treatment.
Source Title: ONCOGENE
ISSN: 09509232
DOI: 10.1038/s41388-021-01661-4
Appears in Collections:Staff Publications

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