Please use this identifier to cite or link to this item: https://doi.org/10.3791/62638
Title: Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases
Authors: Seah, Ivan
LIU ZENGPING 
WONG SOO LIN 
Wong, Wendy
Holder, Graham E
VELUCHAMY AMUTHA BARATHI
LINGAM GOPAL
SU XINYI 
Stanzel, Boris
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
STEM-CELLS
OCRIPLASMIN
EFFICACY
DELIVERY
SAFETY
Issue Date: 1-Jun-2021
Publisher: JOURNAL OF VISUALIZED EXPERIMENTS
Citation: Seah, Ivan, LIU ZENGPING, WONG SOO LIN, Wong, Wendy, Holder, Graham E, VELUCHAMY AMUTHA BARATHI, LINGAM GOPAL, SU XINYI, Stanzel, Boris (2021-06-01). Retinal Pigment Epithelium Transplantation in a Non-human Primate Model for Degenerative Retinal Diseases. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS 2021 (172). ScholarBank@NUS Repository. https://doi.org/10.3791/62638
Abstract: Retinal pigment epithelial (RPE) transplantation holds great promise for the treatment of inherited and acquired retinal degenerative diseases. These conditions include retinitis pigmentosa (RP) and advanced forms of age-related macular degeneration (AMD), such as geographic atrophy (GA). Together, these disorders represent a significant proportion of currently untreatable blindness globally. These unmet medical needs have generated heightened academic interest in developing methods of RPE replacement. Among the animal models commonly utilized for preclinical testing of therapeutics, the non-human primate (NHP) is the only animal model that has a macula. As it shares this anatomical similarity with the human eye, the NHP eye is an important and appropriate preclinical animal model for the development of advanced therapy medicinal products (ATMPs) such as RPE cell therapy. This manuscript describes a method for the submacular transplantation of an RPE monolayer, cultured on a polyethylene terephthalate (PET) cell carrier, underneath the macula onto a surgically created RPE wound in immunosuppressed NHPs. The fovea-the central avascular portion of the macula-is the site of the greatest mechanical weakness during the transplantation. Foveal trauma will occur if the initial subretinal fluid injection generates an excessive force on the retina. Hence, slow injection under perfluorocarbon liquid (PFCL) vitreous tamponade is recommended with a dual-bore subretinal injection cannula at low intraocular pressure (IOP) settings to create a retinal bleb. Pretreatment with an intravitreal plasminogen injection to release parafoveal RPEphotoreceptor adhesions is also advised. These combined strategies can reduce the likelihood of foveal tears when compared to conventional techniques. The NHP is a key animal model in the preclinical phase of RPE cell therapy development. This protocol addresses the technical challenges associated with the delivery of RPE cellular therapy in the NHP eye.
Source Title: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
URI: https://scholarbank.nus.edu.sg/handle/10635/206772
ISSN: 1940-087X
DOI: 10.3791/62638
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