Please use this identifier to cite or link to this item: https://doi.org/10.1007/978-3-030-28471-8_6
Title: Surgical Approaches for Cell Therapeutics Delivery to the Retinal Pigment Epithelium and Retina
Authors: Stanzel, Boris
Ader, Marius
LIU ZENGPING 
Amaral, Juan
Aguirre, Luis Ignacio Reyes
Rickmann, Annekatrin
Barathi, Veluchamy A
Tan, Gavin SW
Degreif, Andrea
Al-Nawaiseh, Sami
Szurman, Peter
Keywords: Science & Technology
Life Sciences & Biomedicine
Cell & Tissue Engineering
Medicine, Research & Experimental
Ophthalmology
Cell Biology
Research & Experimental Medicine
Age-related macular degeneration
Retinal pigment epithelium
Photoreceptor
Transplantation
Cell-based therapy
Cell replacement
Surgery
Anesthesia
Mouse
Rat
Rabbit
Pig
Nonhuman primate
Monkey
Preclinical study
Vitrectomy
Immunosuppression
EMBRYONIC STEM-CELLS
SUBRETINAL IMPLANTATION
VISUAL FUNCTION
RAT MODEL
TRANSPLANTATION
DEGENERATION
RPE
SURVIVAL
SHEETS
RESTORATION
Issue Date: 1-Jan-2019
Publisher: SPRINGER INTERNATIONAL PUBLISHING AG
Citation: Stanzel, Boris, Ader, Marius, LIU ZENGPING, Amaral, Juan, Aguirre, Luis Ignacio Reyes, Rickmann, Annekatrin, Barathi, Veluchamy A, Tan, Gavin SW, Degreif, Andrea, Al-Nawaiseh, Sami, Szurman, Peter (2019-01-01). Surgical Approaches for Cell Therapeutics Delivery to the Retinal Pigment Epithelium and Retina. PLURIPOTENT STEM CELLS IN EYE DISEASE THERAPY 1186 : 141-170. ScholarBank@NUS Repository. https://doi.org/10.1007/978-3-030-28471-8_6
Abstract: Developing successful surgical strategies to deliver cell therapeutics to the back of the eye is an essential pillar to success for stem cell-based applications in blinding retinal diseases. Within this chapter, we have attempted to gather all key considerations during preclinical animal trials. Guidance is provided for choices on animal models, options for immunosuppression, as well as anesthesia. Subsequently we cover surgical strategies for RPE graft delivery, both as suspension as well as in monolayers in small rodents, rabbits, pigs, and nonhuman primate. A detailed account is given in particular on animal variations in vitrectomy and subretinal surgery, which requires a considerable learning curve, when transiting from human to animal. In turn, however, many essential subretinal implantation techniques in large-eyed animals are directly transferrable to human clinical trial protocols. A dedicated subchapter on photoreceptor replacement provides insights on preparation of suspension as well as sheet grafts, to subsequently outline the basics of subretinal delivery via both the transscleral and transvitreal route. In closing, a future outlook on vision restoration through retinal cell-based therapeutics is presented.
Source Title: PLURIPOTENT STEM CELLS IN EYE DISEASE THERAPY
URI: https://scholarbank.nus.edu.sg/handle/10635/206762
ISSN: 0065-2598
2214-8019
DOI: 10.1007/978-3-030-28471-8_6
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