Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2013.12.069
Title: Enhancement of retinal pigment epithelial culture characteristics and subretinal space tolerance of scaffolds with 200 nm fiber topography
Authors: LIU ZENGPING 
Yu, Na
Holz, Frank G
Yang, Fang
Stanzel, Boris V
Keywords: Science & Technology
Technology
Engineering, Biomedical
Materials Science, Biomaterials
Engineering
Materials Science
Biocompatibility
Cell culture
Epithelium cell
Scaffold
Surface topography
Retina
HUMAN BRUCHS MEMBRANE
TISSUE ENGINEERING APPLICATIONS
ANTERIOR LENS CAPSULE
STEM-CELL
MACULAR DEGENERATION
POLYMERIC NANOFIBERS
RPE TRANSPLANTATION
IN-VITRO
DETACHMENT
ADHESION
Issue Date: 1-Mar-2014
Publisher: ELSEVIER SCI LTD
Citation: LIU ZENGPING, Yu, Na, Holz, Frank G, Yang, Fang, Stanzel, Boris V (2014-03-01). Enhancement of retinal pigment epithelial culture characteristics and subretinal space tolerance of scaffolds with 200 nm fiber topography. BIOMATERIALS 35 (9) : 2837-2850. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2013.12.069
Abstract: Tissue engineered retinal pigment epithelial (RPE) transplantation is a promising cell-based therapy for age-related macular degeneration. The aim of this work is to develop a supportive scaffold with a favorable topography to aid functional RPE monolayer maintenance while being tolerated underneath the retina. To this end, films and electrospun substrates with fiber diameters ranging from 200 to 1000nm were made of polyethylene terephthalate or poly(l-lactide-co-ε-caprolactone), and then tested using human fetal RPE cells invitro and transplanted subretinally in rabbits. The results indicated that RPE on both 200nm fiber variants showed the highest cell densities, adherent monolayers achieved deeper pigmentation, and more uniform hexagonal tight junctions. Facile subretinal implantation of flat 200nm fiber membranes was achieved by electrospinning them onto a porous rigid-elastic carrier. Spectral-domain optical coherence tomography showed a reattached, slightly thinned retina overlying the implants over 2 weeks observation. Histology demonstrated native RPE variably migrated onto the nanofibers, and a reactive gliosis with some photoreceptor degeneration. In conclusion, scaffolds with 200nm fiber topography enhanced RPE culture, showed subretinal biocompatibility, and should thus be considered for future cell-based therapies in blinding retinal diseases. © 2014 Elsevier Ltd.
Source Title: BIOMATERIALS
URI: https://scholarbank.nus.edu.sg/handle/10635/206755
ISSN: 0142-9612
1878-5905
DOI: 10.1016/j.biomaterials.2013.12.069
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