Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.stemcr.2013.11.005
Title: Human RPE Stem Cells Grown into Polarized RPE Monolayers on a Polyester Matrix Are Maintained after Grafting into Rabbit Subretinal Space
Authors: Stanzel, Boris V
LIU ZENGPING 
Somboonthanakij, Sudawadee
Wongsawad, Warapat
Brinken, Ralf
Eter, Nicole
Corneo, Barbara
Holz, Frank G
Temple, Sally
Stern, Jeffrey H
Blenkinsop, Timothy A
Keywords: Science & Technology
Life Sciences & Biomedicine
Cell & Tissue Engineering
Cell Biology
RETINAL-PIGMENT EPITHELIUM
MACULAR DEGENERATION
IMMUNE-RESPONSE
TRANSPLANTATION
MODEL
IMMUNOSUPPRESSION
IMPLANTATION
XENOGRAFTS
GENERATION
DETACHMENT
Issue Date: 14-Jan-2014
Publisher: CELL PRESS
Citation: Stanzel, Boris V, LIU ZENGPING, Somboonthanakij, Sudawadee, Wongsawad, Warapat, Brinken, Ralf, Eter, Nicole, Corneo, Barbara, Holz, Frank G, Temple, Sally, Stern, Jeffrey H, Blenkinsop, Timothy A (2014-01-14). Human RPE Stem Cells Grown into Polarized RPE Monolayers on a Polyester Matrix Are Maintained after Grafting into Rabbit Subretinal Space. STEM CELL REPORTS 2 (1) : 64-77. ScholarBank@NUS Repository. https://doi.org/10.1016/j.stemcr.2013.11.005
Abstract: Transplantation of the retinal pigment epithelium (RPE) is being developed as a cell-replacement therapy for age-related macular degeneration. Human embryonic stem cell (hESC) and induced pluripotent stem cell (iPSC)-derived RPE are currently translating toward clinic. We introduce the adult human RPE stem cell (hRPESC) as an alternative RPE source. Polarized monolayers of adult hRPESC-derived RPE grown on polyester (PET) membranes had near-native characteristics. Trephined pieces of RPE monolayers on PET were transplanted subretinally in the rabbit, a large-eyed animal model. After 4 days, retinal edema was observed above the implant, detected by spectral domain optical coherence tomography (SD-OCT) and fundoscopy. At 1 week, retinal atrophy overlying the fetal or adult transplant was observed, remaining stable thereafter. Histology obtained 4 weeks after implantation confirmed a continuous polarized human RPE monolayer on PET. Taken together, the xeno-RPE survived with retained characteristics in the subretinal space. These experiments support that adult hRPESC-derived RPE are a potential source for transplantation therapies.
Source Title: STEM CELL REPORTS
URI: https://scholarbank.nus.edu.sg/handle/10635/206753
ISSN: 2213-6711
DOI: 10.1016/j.stemcr.2013.11.005
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